Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineering
DC Field | Value | Language |
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dc.contributor.author | Gopalakrishnan, Raja Mohan | - |
dc.contributor.author | Manavalan, Tamilvendan | - |
dc.contributor.author | Ramesh, Janani | - |
dc.contributor.author | Thangavelu, Kalaichelvan Puthupalayam | - |
dc.contributor.author | Heese, Klaus | - |
dc.date.accessioned | 2022-07-08T14:06:29Z | - |
dc.date.available | 2022-07-08T14:06:29Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146220 | - |
dc.description.abstract | Trichoderma reesei produces various saccharification enzymes required for biomass degradation. However, the lack of an effective lignin-degrading enzyme system reduces the species' efficiency in producing fermentable sugars and increases the pre-treatment costs for biofuel production. In this study, we heterologously expressed the Ganoderma lucidum RMK1 versatile peroxidase gene (vp1) in the Rut-C30 strain of T. reesei. The expression of purified 6xHis-tag containing recombinant G. lucidum-derived protein (rVP1) was confirmed through western blot, which exhibited a single band with a relative molecular weight of 39 kDa. In saccharification and delignification studies using rice straw, the transformant (tVP7, T. reesei Rut-C30 expressing G. lucidum-derived rVP1) showed significant improvement in the yield of total reducing sugar and delignification, compared with that of the parent T. reesei Rut-C30 strain. Scanning electron microscopy (SEM) of tVP7-treated paddy straw showed extensive degradation of several layers of its surface compared with the parent strain due to the presence of G. lucidum-derived rVP1. Our results suggest that the expression of ligninolytic enzymes in cellulase hyperproducing systems helps to integrate the pre-treatment and saccharification steps that may ultimately reduce the costs of bioethanol production. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineering | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Heese, Klaus | - |
dc.identifier.doi | 10.3390/microorganisms8020159 | - |
dc.identifier.scopusid | 2-s2.0-85078740758 | - |
dc.identifier.wosid | 000519618200015 | - |
dc.identifier.bibliographicCitation | MICROORGANISMS, v.8, no.2, pp.1 - 14 | - |
dc.relation.isPartOf | MICROORGANISMS | - |
dc.citation.title | MICROORGANISMS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ERYNGII VERSATILE PEROXIDASE | - |
dc.subject.keywordPlus | HETEROLOGOUS PROTEIN EXPRESSION | - |
dc.subject.keywordPlus | WHITE-ROT FUNGUS | - |
dc.subject.keywordPlus | RICE STRAW | - |
dc.subject.keywordPlus | ENZYMATIC-HYDROLYSIS | - |
dc.subject.keywordPlus | TRANSFORMATION SYSTEM | - |
dc.subject.keywordPlus | PLEUROTUS-OSTREATUS | - |
dc.subject.keywordPlus | ETHANOL-PRODUCTION | - |
dc.subject.keywordPlus | FILAMENTOUS FUNGI | - |
dc.subject.keywordPlus | LACCASE GENE | - |
dc.subject.keywordAuthor | bioethanol | - |
dc.subject.keywordAuthor | biomass degradation | - |
dc.subject.keywordAuthor | delignification | - |
dc.subject.keywordAuthor | Ganoderma lucidum | - |
dc.subject.keywordAuthor | scanning electron microscope | - |
dc.subject.keywordAuthor | Trichoderma reesei | - |
dc.subject.keywordAuthor | versatile peroxidase | - |
dc.identifier.url | https://www.mdpi.com/2076-2607/8/2/159 | - |
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