Improvement of Saccharification and Delignification Efficiency of Trichoderma reesei Rut-C30 by Genetic Bioengineeringopen access
- Authors
- Gopalakrishnan, Raja Mohan; Manavalan, Tamilvendan; Ramesh, Janani; Thangavelu, Kalaichelvan Puthupalayam; Heese, Klaus
- Issue Date
- Feb-2020
- Publisher
- MDPI
- Keywords
- bioethanol; biomass degradation; delignification; Ganoderma lucidum; scanning electron microscope; Trichoderma reesei; versatile peroxidase
- Citation
- MICROORGANISMS, v.8, no.2, pp.1 - 14
- Indexed
- SCIE
SCOPUS
- Journal Title
- MICROORGANISMS
- Volume
- 8
- Number
- 2
- Start Page
- 1
- End Page
- 14
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146220
- DOI
- 10.3390/microorganisms8020159
- Abstract
- Trichoderma reesei produces various saccharification enzymes required for biomass degradation. However, the lack of an effective lignin-degrading enzyme system reduces the species' efficiency in producing fermentable sugars and increases the pre-treatment costs for biofuel production. In this study, we heterologously expressed the Ganoderma lucidum RMK1 versatile peroxidase gene (vp1) in the Rut-C30 strain of T. reesei. The expression of purified 6xHis-tag containing recombinant G. lucidum-derived protein (rVP1) was confirmed through western blot, which exhibited a single band with a relative molecular weight of 39 kDa. In saccharification and delignification studies using rice straw, the transformant (tVP7, T. reesei Rut-C30 expressing G. lucidum-derived rVP1) showed significant improvement in the yield of total reducing sugar and delignification, compared with that of the parent T. reesei Rut-C30 strain. Scanning electron microscopy (SEM) of tVP7-treated paddy straw showed extensive degradation of several layers of its surface compared with the parent strain due to the presence of G. lucidum-derived rVP1. Our results suggest that the expression of ligninolytic enzymes in cellulase hyperproducing systems helps to integrate the pre-treatment and saccharification steps that may ultimately reduce the costs of bioethanol production.
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