Disease modeling and stem cell immunoengineering in regenerative medicine using CRISPR/Cas9 systemsopen access
- Authors
- Antao, Ainsley Mike; Karapurkar, Janardhan Keshav; Lee, Dong Ryul; Kim, Kye-Seong; Ramakrishna, Suresh
- Issue Date
- Jan-2020
- Publisher
- ELSEVIER
- Keywords
- CRISPR; Cas9; HLA; Disease models; CAR T
- Citation
- COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v.18, pp 3649 - 3665
- Pages
- 17
- Indexed
- SCIE
SCOPUS
- Journal Title
- COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
- Volume
- 18
- Start Page
- 3649
- End Page
- 3665
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/146359
- DOI
- 10.1016/j.csbj.2020.11.026
- ISSN
- 2001-0370
2001-0370
- Abstract
- CRISPR/Cas systems are popular genome editing tools that belong to a class of programmable nucleases and have enabled tremendous progress in the field of regenerative medicine. We here outline the structural and molecular frameworks of the well-characterized type II CRISPR system and several computational tools intended to facilitate experimental designs. The use of CRISPR tools to generate disease models has advanced research into the molecular aspects of disease conditions, including unraveling the molecular basis of immune rejection. Advances in regenerative medicine have been hindered by major histocompatibility complex-human leukocyte antigen (HLA) genes, which pose a major barrier to cell- or tissue-based transplantation. Based on progress in CRISPR, including in recent clinical trials, we hypothesize that the generation of universal donor immune-engineered stem cells is now a realistic approach to tackling a multitude of disease conditions.
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