Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Sojung | - |
dc.contributor.author | Yu, Seungmin | - |
dc.contributor.author | Park, Hye Jeong | - |
dc.contributor.author | Jung, Jiyeon | - |
dc.contributor.author | Go, Gwang-Woong | - |
dc.contributor.author | Kim, Wooki | - |
dc.date.accessioned | 2022-07-09T05:53:27Z | - |
dc.date.available | 2022-07-09T05:53:27Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/147068 | - |
dc.description.abstract | Previous studies have revealed the anti-inflammatory properties of rice bran oil (RBO), but the detailed mechanisms are poorly understood. Recent studies on the molecular/cellular anti-inflammatory mechanisms of dietary components have demonstrated that mitochondrial respiration plays a key role in macrophage functioning. Since dietary lipids are major substrates for mitochondrial respiration through β-oxidation, the current study examined whether RBO regulates inflammatory responses by modulating mitochondrial energy metabolism. Palm oil (PO), enriched with palmitic acid which are known to be effectively taken up by cells and used for oxidative phosphorylation, served as a positive control. In the in vitro model of LPS-stimulated RAW 264.7 murine cells, the levels of pro-inflammatory cytokines (IL-6 and TNF-α) in the culture supernatant were significantly reduced by RBO treatment. In contrast, secretion of the anti-inflammatory cytokine IL-10 was upregulated by RBO. Transcription of genes encoding inflammatory mediator molecules (COX-2 and iNOS) and expression of activation markers (CD80, CD86, and MHC-II) in LPS-stimulated RAW 264.7 cells were suppressed by RBO. Mitochondrial respiration (as assessed by an extracellular flux analyzer) increased upon RBO treatment, as the basal respiration, maximal respiration, ATP production, and spare respiratory capacity were upregulated. In an in vivo study, C57BL/6 mice were fed a negative control diet containing corn oil (CO), PO, or RBO for 4 weeks, and bone marrow-derived macrophages (BMDM) were isolated from their tibias and femurs. In pro-inflammatory M1-polarized BMDM (M1-BMDM), the RBO-induced suppression of IL-6 and TNF-α was recapitulated in vivo. Mitochondrial respiration in M1-BMDM also increased following the RBO intervention and the PO control treatment as compared to CO fed negative control. Overall, the current study for the first time demonstrates that RBO regulates inflammatory responses in murine macrophages by upregulating mitochondrial respiration. Further clinical studies are required to validate the animal study. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Rice bran oil ameliorates inflammatory responses by enhancing mitochondrial respiration in murine macrophages | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Go, Gwang-Woong | - |
dc.identifier.doi | 10.1371/journal.pone.0222857 | - |
dc.identifier.scopusid | 2-s2.0-85073160515 | - |
dc.identifier.wosid | 000532477400007 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.14, no.10, pp.1 - 14 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 14 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | LIPOLYSIS | - |
dc.subject.keywordPlus | BIOLOGY | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | MICE | - |
dc.identifier.url | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0222857 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.