Adenine base editors catalyze cytosine conversions in human cells
- Authors
- Kim, Heon Seok; Jeong, You Kyeong; Hur, Junho K.; Kim, Jin-Soo; Bae, Sangsu
- Issue Date
- Oct-2019
- Publisher
- NATURE RESEARCH
- Citation
- Nature Biotechnology, v.37, no.10, pp.1145 - 1148
- Indexed
- SCIE
SCOPUS
- Journal Title
- Nature Biotechnology
- Volume
- 37
- Number
- 10
- Start Page
- 1145
- End Page
- 1148
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/147099
- DOI
- 10.1038/s41587-019-0254-4
- ISSN
- 1087-0156
- Abstract
- Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing.
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