Metabolic shift of Klebsiella pneumoniae L17 by electrode-based electron transfer using glycerol in a microbial fuel cell
- Authors
- Kim, Mi Yeon; Kim, Changman; Ainala, Satish Kumar; Bae, Hyokwan; Jeon, Byong Hun; Park, Sunghoon; Kim, Jung Rae
- Issue Date
- Feb-2019
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Microbial fuel cell; mRNA expression; RT-PCR; Klebsiella pneumonia L17; Electrode based electron transfer; Metabolic shift; Metabolic flux analysis
- Citation
- BIOELECTROCHEMISTRY, v.125, pp.1 - 7
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOELECTROCHEMISTRY
- Volume
- 125
- Start Page
- 1
- End Page
- 7
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/148347
- DOI
- 10.1016/j.bioelechem.2018.08.002
- ISSN
- 1567-5394
- Abstract
- Klebsiella pneumoniae is used widely for the production of value-added chemicals from glycerol, and is known as an exoelectrogen with an externally provided electron shuttle. In this study, the metabolic shift in K pneumoniae L17 by the activation of electrode-based respiration was examined using microbial fuel cells (MFCs). The mRNA expression levels of the related enzymes for glycerol conversion were compared under electrode-driven anaerobic respirational conditions (i.e., MFC) and fermentative conditions (i.e., non-MFC). mRNA expression clearly responded to the electrode-based electron transfer with simultaneous current generation and changes in metabolite production. The NAD(+)-dependent pathways were activated and more acetate (21.7 vs. 14.6 mM), 3-HP (7.6 vs. 53 mM) and 1,3-PDO (455 vs. 38.1 mM) and less ethanol production were observed under MFC conditions than under non-MFC (39.6 vs 66.7 mM). Stoichiometric metabolic flux analysis was examined in MFC condition. These results suggest that electron excretion to the carbon electrode drives the metabolic pathway shift of K. pneumoniae L17, and can provide an active control strategy for the fermentative pathway of glycerol.
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