Two-Dimensional X-Ray Angiography to Examine Fine Vascular Structure Using a Silicone Rubber Injection Compound
- Authors
- Chang, Hak; Ha, Jeong Hyun; Park, Seong Oh
- Issue Date
- Jan-2019
- Publisher
- JOURNAL OF VISUALIZED EXPERIMENTS
- Keywords
- Medicine; Issue 143; Angiography; silicone rubber injection compound; lead oxide; barium; flap territory; angiosome; radiopaque material; angiographic agent
- Citation
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no.143
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
- Number
- 143
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/148510
- DOI
- 10.3791/57732
- ISSN
- 1940-087X
- Abstract
- Angiography is an essential tool for the study of vascular structures in various research fields. The aim of this study is to introduce a simple angiographic method for examining the fine vascular structure of unfixed, fresh tissue using a silicone rubber injection compound and soft tissue X-ray system. This study is especially focused on flap territories used in reconstructive surgery. This study employs angiography with a silicone rubber injection compound in various experimental conditions using Sprague-Dawley rats. First, 15 mL of MV compound and 15 mL of diluent is mixed. Then, 1.5 mL of the curing agent is prepared, and a 24G catheter is cannulated in the common carotid artery of the rat. A three-way stopcock is then connected to a catheter, and the radiopaque agent, after being mixed with the prepared curing agent, is injected immediately without spillage. Finally, as the agent solidifies, the specimen is harvested, and an angiographic image is obtained using a soft tissue X-ray system. This method indicates that high-quality angiography showing fine vascular structures can be easily and simply obtained within in a short period of time.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 성형외과학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/148510)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.