Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol productionopen access
- Authors
- Waghmare, Pankajkumar R.; Khandare, Rahul V.; Jeon, Byong Hun; Govindwar, Sanjay P.
- Issue Date
- Sep-2018
- Publisher
- GREEN WAVE PUBL CANADA
- Keywords
- Sorghum husk; Phanerochaete chrysosporium; Delignification; Biological pretreatment; Enzymatic hydrolysis; Ethanol production
- Citation
- BIOFUEL RESEARCH JOURNAL-BRJ, v.5, no.3, pp.846 - 853
- Indexed
- SCOPUS
- Journal Title
- BIOFUEL RESEARCH JOURNAL-BRJ
- Volume
- 5
- Number
- 3
- Start Page
- 846
- End Page
- 853
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/149374
- DOI
- 10.18331/BRJ2018.5.3.4
- ISSN
- 2292-8782
- Abstract
- Biological pretreatment of lignocellulosic biomass is considered to be energy-efficient and cost-effective. In the present study, sorghum husk was biologically pretreated with a white-rot fungus Phanerochaete chrysosporium (MTCC 4955) under submerged static condition. Ligninolytic enzymes like lignin peroxidase (0.843 U/mL) and manganese peroxidase (0.389 U/mL) played an important role in the biological pretreatment of sorghum husk. Activities of different hydrolytic enzymes such as endoglucanase (57.25 U/mL), exoglucanase (4.76 U/mL), filter paperase (0.580 U/mL), glucoamylase (153.38 U/mL), and xylanase (88.14 U/mL) during biological pretreatment of sorghum husk by P. chrysosporium were evaluated. Enzymatic hydrolysis of untreated sorghum husk and biologically pretreated sorghum husk produced 20.07 and 103.0 mg/g reducing sugars, respectively. This result showed a significant increase in reducing sugar production in the biologically pretreated sorghum husk as compared to its untreated counterpart. Biologically pretreated sorghum husk hydrolysate was further fermented for 48 h using Saccharomyces cerevisiae (KCTC 7296), Pachysolen tannophilus (MTCC 1077), and their co-culture resulting in ethanol yields of 2.113, 1.095, and 2.348%, respectively. The surface characteristics of the substrate were evaluated after the delignification and hydrolysis, using FTIR, XRD, and SEM, confirming the effectiveness of the biological pretreatment process.
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