Pumpless microflow cytometry enabled by viscosity modulation and immunobead labeling
- Authors
- Kim, Byeongyeon; Oh, Sein; Shin, Suyeon; Yim, Sang-Gu; Yang, Seung Yun; Hahn, Young Ki; Choi, Sung young
- Issue Date
- Jul-2018
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Flow cytometry; Microparticles; Fluorescence; Cells; Viscosity
- Citation
- ANALYTICAL CHEMISTRY, v.90, no.13, pp.8254 - 8260
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 90
- Number
- 13
- Start Page
- 8254
- End Page
- 8260
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/149678
- DOI
- 10.1021/acs.analchem.8b01804
- ISSN
- 0003-2700
- Abstract
- Major challenges of miniaturizing flow cytometry include obviating the need for bulky, expensive, and complex pump-based fluidic and laser-based optical systems while retaining the ability to detect target cells based on their unique surface receptors. We addressed these critical challenges by (i) using a viscous liquid additive to control flow rate passively, without external pumping equipment, and (ii) adopting an immunobead assay that can be quantified with a portable fluorescence cell counter based on a blue light-emitting diode. Such novel features enable pumpless microflow cytometry (pFC) analysis by simply dropping a sample solution onto the inlet reservoir of a disposable cell-counting chamber. With our pFC platform, we achieved reliable cell counting over a dynamic range of 9–298 cells/μL. We demonstrated the practical utility of the platform by identifying a type of cancer cell based on CD326, the epithelial cell adhesion molecule. This portable microflow cytometry platform can be applied generally to a range of cell types using immunobeads labeled with specific antibodies, thus making it valuable for cell-based and point-of-care diagnostics.
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