Comparison of human umbilical cord blood-derived mesenchymal stem cells with healthy fibroblasts on wound-healing activity of diabetic fibroblastsopen access
- Authors
- Jung, Jae-A; Yoon, Young-Don; Lee, Hyup-Woo; Kang, So-Ra; Han, Seung-Kyu
- Issue Date
- Feb-2018
- Publisher
- WILEY
- Keywords
- diabetic wound healing; fibroblast; human umbilical cord blood stromal cells
- Citation
- INTERNATIONAL WOUND JOURNAL, v.15, no.1, pp.133 - 139
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL WOUND JOURNAL
- Volume
- 15
- Number
- 1
- Start Page
- 133
- End Page
- 139
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/150537
- DOI
- 10.1111/iwj.12849
- ISSN
- 1742-4801
- Abstract
- Various types of skin substitutes composed of fibroblasts and/or keratinocytes have been used for the treatment of diabetic ulcers. However, the effects have generally not been very dramatic. Recently, human umbilical cord blood-derived mesenchymal stromal cells (hUCB-MSCs) have been commercialised for cartilage repair as a first cell therapy product using allogeneic stem cells. In a previous pilot study, we reported that hUCB-MSCs have a superior wound-healing capability compared with fibroblasts. The present study was designed to compare the treatment effect of hUCB-MSCs with that of fibroblasts on the diabetic wound healing in vitro. Diabetic fibroblasts were cocultured with healthy fibroblasts or hUCB-MSCs. Five groups were evaluated: group I, diabetic fibroblasts without coculture; groups II and III, diabetic fibroblasts cocultured with healthy fibroblasts or hUCB-MSCs; and groups IV and V, no cell cocultured with healthy fibroblasts or hUCB-MSCs. After a 3-day incubation, cell proliferation, collagen synthesis levels and glycosaminoglycan levels, which are the major contributing factors in wound healing, were measured. As a result, a hUCB-MSC-treated group showed higher cell proliferation, collagen synthesis and glycosaminoglycan level than a fibroblast-treated group. In particular, there were significant statistical differences in collagen synthesis and glycosaminoglycan levels (P = 0029 and P = 0019, respectively). In conclusion, these results demonstrate that hUCB-MSCs may have a superior effect to fibroblasts in stimulating diabetic wound healing.
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