Targeted knockout of phospholipase A2 to increase lipid productivity in Chlamydomonas reinhardtii for biodiesel production
- Authors
- Shin, Ye Sol; Jeong, Jooyeon; Nguyen, Thu Ha Thi; Kim, Jaoon Young Hwan; Jin, EonSeon; Sim, Sang Jun
- Issue Date
- Jan-2019
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Chlamydomonas reinhardtii; Biofuel; Triacylglycerol; Phospholipase; CRISPR-CAS9
- Citation
- BIORESOURCE TECHNOLOGY, v.271, pp.368 - 374
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 271
- Start Page
- 368
- End Page
- 374
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/15123
- DOI
- 10.1016/j.biortech.2018.09.121
- ISSN
- 0960-8524
- Abstract
- Biofuel derived from microalgae have several advantages over other oleaginous crops, however, still needs to be improved with its cost aspect and can be achieved by developing of a strain with improved lipid productivity. In this study, the CRISPR-Cas9 system was incorporated to carry out a target-specific knockout of the phospholipase A2 gene in Chlamydomonas reinhardtii. The targeted gene encodes a key enzyme in the Lands cycle. As a result, the mutants showed a characteristic of increased diacylglycerol pool, followed by a higher accumulation of triacylglycerol without being significantly compensated with the cell growth. As a result, the overall lipid productivities of phospholipase A2 knockout mutants have increased by up to 64.25% (to 80.92 g L−1 d−1). This study can provide crucial information for the biodiesel industry.
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