Feasibility and Stability of Liver Biopsy before Treatment for Preclinical Nonalcoholic Fatty Liver Studies
DC Field | Value | Language |
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dc.contributor.author | Chae, Yeon Ji | - |
dc.contributor.author | Jun, Dae Won | - |
dc.contributor.author | Saeed, Waqar Khalid | - |
dc.contributor.author | Kang, Hyeon Tae | - |
dc.contributor.author | Oh, Ju Hee | - |
dc.contributor.author | Lee, Seung Min | - |
dc.contributor.author | Jang, Kiseok | - |
dc.date.accessioned | 2021-08-02T12:28:41Z | - |
dc.date.available | 2021-08-02T12:28:41Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/15152 | - |
dc.description.abstract | Background: The heterogeneity of histological findings in preclinical diet-induced nonalcoholic fatty liver disease (NAFLD) animal models is highly challenging. Here, we aimed to evaluate the feasibility and stability of repeated liver biopsy in NAFLD animal models. Methods: Heterogeneity of diet-induced NAFLD was evaluated at different time points in 52 high-fat diet (HFD), 35 methionine choline-deficiency diet (MCD), and 166 western diet (WD) induced NAFLD mice. Serial liver biopsies (left lateral, right medial, and left medial lobes) were performed monthly for up to 3 months. Mortality rates and changes in food intake, body weight, and liver enzymes were assessed. Results: At 12 weeks, of the HFD animals, 14% and 30% did not develop steatosis and lobular inflammation, respectively; of the MCD animals, 7% did not develop lobular inflammation; and of the WD animals, 14% and 51% did not develop steatosis and lobular inflammation, respectively. The mortality rate of repeated liver biopsy was 1.62% (2/123 mice died). Repeated liver biopsy can be used to trace disease progression. Although body weight, food intake, and liver enzymes slightly changed after biopsy, all recovered within a week. Repeated liver biopsy did not affect the degrees of inflammation and steatosis of the other liver lobes. Conclusion: The diet-induced NAFLD models were quite heterogeneous. Our results suggest that the repeated liver biopsy before treatment was applicable and stable in this NAFLD animal study. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN ACAD MEDICAL SCIENCES | - |
dc.title | Feasibility and Stability of Liver Biopsy before Treatment for Preclinical Nonalcoholic Fatty Liver Studies | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jun, Dae Won | - |
dc.contributor.affiliatedAuthor | Jang, Kiseok | - |
dc.identifier.doi | 10.3346/jkms.2019.34.e14 | - |
dc.identifier.scopusid | 2-s2.0-85059885831 | - |
dc.identifier.wosid | 000455718600005 | - |
dc.identifier.bibliographicCitation | Journal of Korean Medical Science, v.34, no.2, pp.1 - 11 | - |
dc.relation.isPartOf | Journal of Korean Medical Science | - |
dc.citation.title | Journal of Korean Medical Science | - |
dc.citation.volume | 34 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002432648 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | General & Internal Medicine | - |
dc.relation.journalWebOfScienceCategory | Medicine, General & Internal | - |
dc.subject.keywordPlus | MOUSE MODELS | - |
dc.subject.keywordPlus | OBETICHOLIC ACID | - |
dc.subject.keywordPlus | DIET | - |
dc.subject.keywordPlus | STEATOHEPATITIS | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | ELAFIBRANOR | - |
dc.subject.keywordPlus | C57BL/6J | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordAuthor | Animal Model | - |
dc.subject.keywordAuthor | Nonalcoholic Fatty Liver Disease | - |
dc.subject.keywordAuthor | Biopsy | - |
dc.identifier.url | https://jkms.org/DOIx.php?id=10.3346/jkms.2019.34.e14 | - |
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