Genome editing: a robust technology for human stem cells
- Authors
- Chandrasekaran, Arun, Pandian; Song, Minjung; Ramakrishna, Suresh
- Issue Date
- Sep-2017
- Publisher
- SPRINGER BASEL AG
- Keywords
- Induced pluripotent stem cells; Embryonic stem cells; Zinc finger nucleases; Transcription activator-like effector nucleases; Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system
- Citation
- CELLULAR AND MOLECULAR LIFE SCIENCES, v.74, no.18, pp.3335 - 3346
- Indexed
- SCIE
SCOPUS
- Journal Title
- CELLULAR AND MOLECULAR LIFE SCIENCES
- Volume
- 74
- Number
- 18
- Start Page
- 3335
- End Page
- 3346
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/151678
- DOI
- 10.1007/s00018-017-2522-0
- ISSN
- 1420-682X
- Abstract
- Human pluripotent stem cells comprise induced pluripotent and embryonic stem cells, which have tremendous potential for biological and therapeutic applications. The development of efficient technologies for the targeted genome alteration of stem cells in disease models is a prerequisite for utilizing stem cells to their full potential. Genome editing of stem cells is possible with the help of synthetic nucleases that facilitate site-specific modification of a gene of interest. Recent advances in genome editing techniques have improved the efficiency and speed of the development of stem cells for human disease models. Zinc finger nucleases, transcription activator- like effector nucleases, and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system are powerful tools for editing DNA at specific loci. Here, we discuss recent technological advances in genome editing with site-specific nucleases in human stem cells.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의생명공학전문대학원 > 서울 의생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/151678)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.