Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugsopen access
- Authors
- Ahn, Seong Joon; Hong, Hye Kyoung; Na, Young Mi; Park, Sang Jun; Ahn, Jeeyun; Oh, Jaeseong; Chung, Jae Yong; Park, Kyu Hyung; Woo, Se Joon
- Issue Date
- Jul-2016
- Publisher
- JOURNAL OF VISUALIZED EXPERIMENTS
- Keywords
- Medicine; Issue 113; drug; eye; intraocular; intravitreal; pharmacokinetics; rabbit
- Citation
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, no.113
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
- Number
- 113
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154294
- DOI
- 10.3791/53878
- ISSN
- 1940-087X
- Abstract
- The intraocular route of drug administration enables the delivery of high concentrations of therapeutic drugs, while minimizing their systemic absorption. Several drugs are administered into the anterior chamber or vitreous, and the intraocular injection has been effective in curing various intraocular diseases. Rabbit eyes have been widely used for ophthalmic research, as the animal is easy to handle and economical compared to other mammals, and the size of a rabbit eye is similar to that of a human eye. Using a 30 G needle, drugs can be injected into the intracameral and intravitreal spaces of rabbit eyes. The eyeballs are then frozen until analysis, and can be divided into the aqueous humor, vitreous, and retina/choroid. The vitreous and retina/choroid samples can be homogenized and solubilized before analysis. Then, immunoassays can be performed to measure the concentrations of intraocular drugs in each compartment. Appropriate pharmacokinetic models can be used to calculate several parameters, such as the half-life and maximum concentration of the drug. Rabbit eyes can be a good model for pharmacokinetic studies of intraocular drugs.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 안과학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/154294)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.