복막염 환자에서 분리된 Bacteroides nordii 1예와 Bacteroides salyersiae 2예Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients
- Other Titles
- Bacteroides nordii and Bacteroides salyersiae Isolated from Post-operative Peritonitis Patients
- Authors
- 윤지훈; 김현수; 이양순; 김명숙; 용동은; 정석훈; 이경원
- Issue Date
- 2016
- Publisher
- 대한진단검사의학회
- Keywords
- Bacteroides fragilis group; Bacteroides nordii; Bacteroides salyersiae; Spectrometry; Mass; Matrix-assisted laser desorption-ionization
- Citation
- Laboratory Medicine Online, v.6, no.2, pp.111 - 115
- Indexed
- KCI
- Journal Title
- Laboratory Medicine Online
- Volume
- 6
- Number
- 2
- Start Page
- 111
- End Page
- 115
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/155462
- ISSN
- 2093-6338
- Abstract
- 저자들은 수술 후 발열과 함께 복막염이 의심되는 환자에서 시행한 복막액 배양에서 세 건의 드문 Bacteroides 균종을 분리하였다. 세 균주들은 Rapid ID 32A (bioMérieux, France) 검사로 각각 B. fragilis/B. stercoris와 B. ovatus로 동정되었으나, MALDI-TOF MS와 16S rRNA 염기서열 분석을 통해 B. nordii 1예와 B. salyersiae 2예로 확인되었다. 세 균주는 항혐기성 약제인 piperacillin/tazobactam, imipenem, meropenem, chloramphenicol 및 metronidazole에 대해서 모두 감수성이었으며, 환자들은 piperacillin/tazobactam 치료 후 호전되었다. 본 증례를 통해 드문 B. fragilis군균종 동정시에 MALDI-TOF MS가 16S rRNA 염기서열 분석 결과와 일치도가 높고, 간편하고 신속하게 결과를 확인할 수 있다는 점에서 유용함을 알 수 있었다.
In this study, we report three cases in which two species of the Bacteroides fragilis group, ‘Bacteroides nordii’ and ‘Bacteroides salyersiae’, were isolated from peritoneal fluid cultures from post-operative peritonitis patients. The two species of the B. fragilis group were initially misidentified as B. fragilis/Bacteroides stercoris and Bacteroides ovatus by Rapid ID 32A (bioMérieux, France), and finally confirmed as B. nordii and B. salyersiae using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and 16s rRNA sequencing. For the identification of anaerobes, particularly B. fragilis group organisms, MALDI-TOF MS is a useful method not only because of its concordance with 16S rRNA sequencing results, but also because of its rapidity and simple procedure.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 진단검사의학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/155462)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.