CRISPR/Cas9 system as an innovative genetic engineering tool: Enhancements in sequence specificity and delivery methods
- Authors
- Jo, Young-Il; Suresh, Bharathi; Kim, Hyongbum; Ramakrishna, Suresh
- Issue Date
- Dec-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Delivery methods; Genome editing; Reduced off-target effects; Sequence specificity; Therapeutics; CRISPR/Cas9 applications
- Citation
- BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, v.1856, no.2, pp.234 - 243
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
- Volume
- 1856
- Number
- 2
- Start Page
- 234
- End Page
- 243
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/155701
- DOI
- 10.1016/j.bbcan.2015.09.003
- ISSN
- 0304-419X
- Abstract
- While human gene therapy has gained significant attention for its therapeutic promise, CRISPR/Cas9 technology has made a breakthrough as an efficient genome editing tool by emulating prokaryotic immune defense mechanisms. Although many studies have found that CRISPR/Cas9 technology is more efficient, specific and manipulable than previous generations of gene editing tools, it can be further improved by elevating its overall efficiency in a higher frequency of genome modifications and reducing its off-target effects. Here, we review the development of CRISPR/Cas9 technology, focusing on enhancement of its sequence specificity, reduction of off-target effects and delivery systems. Moreover, we describe recent successful applications of CRISPR/Cas9 technology in laboratory and clinical studies.
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