Supplementation of Growth Differentiation Factor-5 Increases Proliferation and Size of Chondrogenic Pellets of Human Umbilical Cord-Derived Perivascular Stem Cells
- Authors
- An, Borim; Heo, Hye-Ryeon; Lee, Sungeun; Park, Jeong-Ah; Kim, Kye-Seong; Yang, Jeonghee; Hong, Seok-Ho
- Issue Date
- Jun-2015
- Publisher
- KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC
- Keywords
- Perivascular stem cells; Growth differentiation factor-5; Proliferation; Chondrocytes
- Citation
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.12, no.3, pp.181 - 187
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- TISSUE ENGINEERING AND REGENERATIVE MEDICINE
- Volume
- 12
- Number
- 3
- Start Page
- 181
- End Page
- 187
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/157099
- DOI
- 10.1007/s13770-015-0113-4
- ISSN
- 1738-2696
- Abstract
- In recent years, perivascular stem cells (PVCs) have gained increasing attention as a promising source for regenerative medicine due to their greater differentiation potential compared to multipotent mesenchymal stem cells (MSCs). It has been reported that growth differentiation factor-5 (GDF-5) is involved in regulating proliferation and chondrogenic differentiation of MSCs. In this study, we investigated the effect of GDF-5 on the proliferation and chondrogenic differentiation of PVCs isolated from human umbilical cords. The supplementation of PVC cultures with GDF-5 (100 ng/mL) significantly enhanced their proliferative rate and augmented the size of pellets in micromass pellet cultures for chondrogenic induction. Although similar expression levels of chondrogenic-related genes were observed in chondrogenic pellets treated with GDF-5 compared to the pellets without GDF-5 treatment, these results indicate that supplementation of GDF-5 is able to acquire more chondrocytes when starting with equal amount of PVCs. Our study suggests that GDF-5 is an effective agent for the enhancement of PVC proliferation, thereby achieving a higher number of chondrocytes that are applicable in therapeutic doses for cartilage regeneration.
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