Effective Gene Delivery into Human Stem Cells with a Cell-Targeting Peptide-Modified Bioreducible Polymer
- Authors
- Beloor, Jagadish; Ramakrishna, Suresh; Nam, Kihoon; Choi, Chang Seon; Kim, Jongkil; Kim, Sung Hwa; Cho, Hyong Jin; Shin, HeungSoo; Kim, Hyongbum; Kim, Sung Wan; Lee, Sang-Kyung; Kumar, Priti
- Issue Date
- May-2015
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- SMALL, v.11, no.17, pp.2069 - 2079
- Indexed
- SCIE
SCOPUS
- Journal Title
- SMALL
- Volume
- 11
- Number
- 17
- Start Page
- 2069
- End Page
- 2079
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/157337
- DOI
- 10.1002/smll.201402933
- ISSN
- 1613-6810
- Abstract
- Stem cells are poorly permissive to non-viral gene transfection reagents. In this study, we explored the possibility of improving gene delivery into human embryonic (hESC) and mesenchymal (hMSC) stem cells by synergizing the activity of a cell-binding ligand with a polymer that releases nucleic acids in a cytoplasm-responsive manner. A 29 amino acid long peptide, RVG, targeting the nicotinic acetylcholine receptor (nAchR) was identified to bind both hMSC and H9-derived hESC. Conjugating RVG to a redox-sensitive biodegradable dendrimer-type arginine-grafted polymer (PAM-ABP) enabled nanoparticle formation with plasmid DNA without altering the environment-sensitive DNA release property and favorable toxicity profile of the parent polymer. Importantly, RVG-PAM-ABP quantitatively enhanced transfection into both hMSC and hESC compared to commercial transfection reagents like Lipofectamine 2000 and Fugene. similar to 60% and 50% of hMSC and hESC were respectively transfected, and at increased levels on a per cell basis, without affecting pluripotency marker expression. RVG-PAM-ABP is thus a novel bioreducible, biocompatible, non-toxic, synthetic gene delivery system for nAchR-expressing stem cells. Our data also demonstrates that a cell-binding ligand like RVG can cooperate with a gene delivery system like PAM-ABP to enable transfection of poorly-permissive cells.
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Collections - 서울 공과대학 > 서울 생명공학과 > 1. Journal Articles
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