Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Chlorpyrifos-induced changes in the antioxidants and fatty acid compositions of Chroococcus turgidus NTMS12open access

Authors
Kumar, M. S.Praveenkumar, R.Jeon, Byong HunThajuddin, N.
Issue Date
Nov-2014
Publisher
WILEY
Keywords
catalase; chlorpyrifos; Chroococcus turgidus; gas chromatography; proline; superoxide dismutase
Citation
LETTERS IN APPLIED MICROBIOLOGY, v.59, no.5, pp.535 - 541
Indexed
SCIE
SCOPUS
Journal Title
LETTERS IN APPLIED MICROBIOLOGY
Volume
59
Number
5
Start Page
535
End Page
541
URI
https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/158709
DOI
10.1111/lam.12311
ISSN
0266-8254
Abstract
This study investigated the effect of pesticide chlorpyrifos (CP) on a freshwater cyanobacterium Chroococcus turgidus NTMS12. The changes in chlorophyll-a, proline, superoxide dismutase (SOD), catalase (CAT) activities and fatty acid composition of the test organism were analysed. Organism was grown at 6, 9 and 12mgl(-1) of CP, and based on the chlorophyll-a content, 6mgl(-1) of CP was found to be the tolerable concentration. Hence, 6mgl(-1) of CP was taken to evaluate the concentration of proline and activities of SOD and CAT at 48-h exposure. The changes in the fatty acid profile were analysed after 7days of exposure. Upon pesticide exposure, increased concentration of proline and activities of SOD and CAT were found. Significant changes in fatty acid profile have also been observed. However, polyunsaturated fatty acid content was decreased in treated cultures when compared with the untreated control. Changes in biochemical activities indicate that cyanobacteria C.turgidus NTMS12 undergo adaptive changes against CP-induced oxidative stress. Significance and Impact of the StudyThe fish pathogenic bacteria of genus Aeromonas, Vibrio, Edwardsiella and Streptococcus frequently cause severe outbreaks of diseases in cultured fish, and the genus-specific multiplex PCR assay developed in this study can detect the bacteria of the four genera when present in the samples either alone or mixed. The mPCR assay is expected to identify the causative agents more efficiently than uniplex PCR or species-specific multiplex PCR for clinical diagnosis, resulting in the earlier implementation of control measures. This mPCR assay provides a rapid, specific and sensitive tool for the detection or identification of common fish pathogenic bacteria in aquaculture practice.
Files in This Item
Appears in
Collections
서울 공과대학 > 서울 자원환경공학과 > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Jeon, Byong Hun photo

Jeon, Byong Hun
COLLEGE OF ENGINEERING (DEPARTMENT OF EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING)
Read more

Altmetrics

Total Views & Downloads

BROWSE