Osthol attenuates hepatic steatosis via decreased triglyceride synthesis not by insulin resistance
- Authors
- Nam, Ho Hyun; Jun, Dae Won; Jeon, Hye Joon; Lee, Jai Sun; Saeed, Waqar Khalid; Kim, Eun Kyung
- Issue Date
- Sep-2014
- Publisher
- BAISHIDENG PUBLISHING GROUP INC
- Keywords
- Osthol; Non-alcoholic fatty liver disease; Sterol regulatory element binding protein
- Citation
- WORLD JOURNAL OF GASTROENTEROLOGY, v.20, no.33, pp.11753 - 11761
- Indexed
- SCIE
SCOPUS
- Journal Title
- WORLD JOURNAL OF GASTROENTEROLOGY
- Volume
- 20
- Number
- 33
- Start Page
- 11753
- End Page
- 11761
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/159247
- DOI
- 10.3748/wjg.v20.i33.11753
- ISSN
- 1007-9327
- Abstract
- AIM: To evaluate the effects of osthol on intrahepatic fat synthesis, beta-oxidation, inflammation, and insulin resistance by multifaceted analysis. METHODS: Sprague-Dawley rats (n = 30) were randomly divided into control, non-alcoholic fatty liver disease (NAFLD), and osthol groups. NAFLD and osthol groups were fed with a high-fat diet for 14 wk. After 8 wk of the high-fat diet, the osthol group also received osthol 20 mg/kg orally 5 times/wk. To assess the insulin resistance, oral glucose tolerance was performed at the end of 14 wk. Immunohistochemical (4-HNE, F4/80) and hematoxylin and eosin (HE) staining were performed on liver tissue extracts after animal sacrifice at 14 wk. SREBP1c, FAS, SCD-1, PPAR-alpha, CROT, MCP-1, IRS-1, and IRS-2 mRNA expressions were assessed with reverse transcription-polymerase chain reaction. RESULTS: HE staining revealed that, compared with the NAFLD group, the osthol group showed significantly decreased intrahepatic fat content (39.4% vs 21.0%; P = 0.021). SREBP1c expression in the NAFLD group increased compared to controls (P = 0.0001), while osthol treatment decreased SREBP1c expression compared with the NAFLD group (P = 0.0059). In the osthol group, intrahepatic FAS and SCD-1, which act downstream of SREBP1c, decreased significantly compared with the NAFLD group. Moreover, PPAR-alpha expression in the osthol group was also significantly higher than in the NAFLD group (P = 0.0147). CONCLUSION: Osthol treatment attenuated liver steatosis by decreasing de novo liver triglyceride synthesis and had nominal effects on insulin resistance and liver inflammation.
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