Spontaneously Differentiated GATA6-Positive Human Embryonic Stem Cells Represent an Important Cellular Step in Human Embryonic Development; They Are Not Just an Artifact of In Vitro Culture
DC Field | Value | Language |
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dc.contributor.author | Lee, Jun Ho | - |
dc.contributor.author | Hong, Ki Sung | - |
dc.contributor.author | Mantel, Charlie | - |
dc.contributor.author | Broxmeyer, Hal E. | - |
dc.contributor.author | Lee, Man Ryul | - |
dc.contributor.author | Kim, Kye-Seong | - |
dc.date.accessioned | 2022-07-16T08:13:58Z | - |
dc.date.available | 2022-07-16T08:13:58Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-10 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/161867 | - |
dc.description.abstract | In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hESCs by their higher expression of GATA6, a marker for primitive endoderm and transthyretin, a marker visceral endoderm in embryoid bodies (EBs). SD-hESCs expressed OCT4 and NANOG, markers for pluripotent stem cells, at significantly lower levels than undifferentiated hESCs. EBs derived from isolated SD-hESCs were morphologically distinct from cells directly derived from the undifferentiated hESCs; they contained higher number of cysts compared to EBs from undifferentiated hESC-derived EBs (42% vs. 20%). Furthermore, the extracellular signal molecule, BMP2/4, induced a higher GATA4/6 expression and cystic EB formation than control and noggin-treated EBs. Since cystic formation in EBs play a role in primitive endoderm formation during embryogenesis, the SD-hESC may be a relevant cell type equipped to differentiate into primitive endoderm. Our results suggest that SD-ESCs generated during routine hESC culture are not just an artifact of in vitro culture and these cells could serve as a useful model to study the process of embryogenesis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Spontaneously Differentiated GATA6-Positive Human Embryonic Stem Cells Represent an Important Cellular Step in Human Embryonic Development; They Are Not Just an Artifact of In Vitro Culture | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kye-Seong | - |
dc.identifier.doi | 10.1089/scd.2013.0083 | - |
dc.identifier.scopusid | 2-s2.0-84885150714 | - |
dc.identifier.wosid | 000325148800003 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, v.22, no.20, pp.2706 - 2713 | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.citation.title | STEM CELLS AND DEVELOPMENT | - |
dc.citation.volume | 22 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 2706 | - |
dc.citation.endPage | 2713 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | VISCERAL ENDODERM | - |
dc.subject.keywordPlus | MOUSE EMBRYO | - |
dc.subject.keywordPlus | GATA FACTORS | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | PLURIPOTENCY | - |
dc.subject.keywordPlus | NICHE | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | BLASTOCYSTS | - |
dc.subject.keywordPlus | CAVITATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.identifier.url | https://www.liebertpub.com/doi/10.1089/scd.2013.0083 | - |
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