Spontaneously Differentiated GATA6-Positive Human Embryonic Stem Cells Represent an Important Cellular Step in Human Embryonic Development; They Are Not Just an Artifact of In Vitro Culture
- Authors
- Lee, Jun Ho; Hong, Ki Sung; Mantel, Charlie; Broxmeyer, Hal E.; Lee, Man Ryul; Kim, Kye-Seong
- Issue Date
- Oct-2013
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- STEM CELLS AND DEVELOPMENT, v.22, no.20, pp.2706 - 2713
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELLS AND DEVELOPMENT
- Volume
- 22
- Number
- 20
- Start Page
- 2706
- End Page
- 2713
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/161867
- DOI
- 10.1089/scd.2013.0083
- ISSN
- 1547-3287
- Abstract
- In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hESCs by their higher expression of GATA6, a marker for primitive endoderm and transthyretin, a marker visceral endoderm in embryoid bodies (EBs). SD-hESCs expressed OCT4 and NANOG, markers for pluripotent stem cells, at significantly lower levels than undifferentiated hESCs. EBs derived from isolated SD-hESCs were morphologically distinct from cells directly derived from the undifferentiated hESCs; they contained higher number of cysts compared to EBs from undifferentiated hESC-derived EBs (42% vs. 20%). Furthermore, the extracellular signal molecule, BMP2/4, induced a higher GATA4/6 expression and cystic EB formation than control and noggin-treated EBs. Since cystic formation in EBs play a role in primitive endoderm formation during embryogenesis, the SD-hESC may be a relevant cell type equipped to differentiate into primitive endoderm. Our results suggest that SD-ESCs generated during routine hESC culture are not just an artifact of in vitro culture and these cells could serve as a useful model to study the process of embryogenesis.
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