Isolation and Characterization of Novel, Highly Proliferative Human CD34/CD73-Double-Positive Testis-Derived Stem Cells for Cell Therapy
- Authors
- Choi, Won Yun; Jeon, Hwang Gyun; Chung, Young; Lim, Jung Jin; Shin, Dong Hyuk; Kim, Jung Mo; Ki, Byeong Seong; Song, Seung-Hun; Choi, Seong-Jun; Park, Keun-Hong; Shim, Sung Han; Moon, Jisook; Jung, Sung Jun; Kang, Hyun Mi; Park, Seah; Chung, Hyung Min; Ko, Jung Jae; Cha, Kwang Yul; Yoon, Tae Ki; Kim, Haekwon; Lee, Dong Ryul
- Issue Date
- Aug-2013
- Publisher
- MARY ANN LIEBERT, INC
- Citation
- STEM CELLS AND DEVELOPMENT, v.22, no.15, pp.2158 - 2173
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELLS AND DEVELOPMENT
- Volume
- 22
- Number
- 15
- Start Page
- 2158
- End Page
- 2173
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162223
- DOI
- 10.1089/scd.2012.0385
- ISSN
- 1547-3287
- Abstract
- Human adult stem cells are a readily available multipotent cell source that can be used in regenerative medicine. Despite many advantages, including low tumorigenicity, their rapid senescence and limited plasticity have curtailed their use in cell-based therapies. In this study, we isolated CD34/CD73-double-positive (CD34(+)/CD73(+)) testicular stromal cells (HTSCs) and found that the expression of CD34 was closely related to the cells' stemness and proliferation. The CD34(+)/CD73(+) cells grew in vitro for an extended period of time, yielding a multitude of cells (5.6 x 10(16) cells) without forming tumors in vivo. They also differentiated into all three germ layer lineages both in vitro and in vivo, produced cartilage more efficiently compared to bone marrow stem cells and, importantly, restored erectile function in a cavernous nerve crush injury rat model. Thus, these HTSCs may represent a promising new autologous cell source for clinical use.
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