Comparison of the clinical performance of restriction fragment mass polymorphism (RFMP) and Roche linear array HPV test assays for HPV detection and genotyping
- Authors
- Lee, Hyo-Pyo; Cho, Woojae; Bae, Jae man; Shin, Ji Young; Shin, Soo-Kyung; Hwang, Sun Young; Min, Kyung Tae; Kim, Soo Nyung; Lee, Sun Joo; Kim, Soo-Ok; Yoo, Wang Don; Hong, Sun Pyo
- Issue Date
- Jun-2013
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Clinical; Genotype; Human papillomavirus; Mass spectrometry
- Citation
- JOURNAL OF CLINICAL VIROLOGY, v.57, no.2, pp.130 - 135
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CLINICAL VIROLOGY
- Volume
- 57
- Number
- 2
- Start Page
- 130
- End Page
- 135
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/162475
- DOI
- 10.1016/j.jcv.2013.01.014
- ISSN
- 1386-6532
- Abstract
- Background: The need for accurate genotyping of human papillomavirus (HPV) infections is becoming increasingly important as HPV is the primary cause of cervical cancer worldwide. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based restriction fragment mass polymorphism (RFMP) assay provides accurate, broad-spectrum, high-throughput genotyping of HPV. Objectives: We evaluated the clinical performance of the RFMP assay compared to a commercially available Roche linear array HPV genotyping test (LA) for detecting and genotyping of HPV. Study design: The RFMP assay and the LA were compared for detecting and genotyping HPV among a cohort of 244 liquid-based cytology samples. Results: Overall, 216 specimens (93.1%, kappa = 0.86) generated concordant results for the presence or absence of high-risk HPV (HR-HPV) by the two assays. The RFMP assay and the LA assay generated concordant, compatible, and discordant genotyping results for 79.3, 9.9, and 10.8%, respectively. The diagnostic sensitivity and specificity of RFMP and LA for the cervical lesions of squamous cell carcinoma (SCC) were similar, at 92.9 and 85.0% (RFMP) and 92.9 and 83.8% (LA), respectively. In addition, the odds ratio for SCC with HR-HPV positivity estimated by the RFMP assay (73.7, 95% CI: 8.9-3173.3) was higher than the LA assay (67.0, 95% CI: 8.2-2887.0). Conclusions: The RFMP and the LA assays were highly comparable with regard to detection and genotyping analysis of HPV. The sensitivity and specificity of RFMP assay for the detection of HR-HPV in various levels of cervical lesions seems to be valuable in the monitoring of HPV-associated cervical cancer.
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