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Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina
- Issue Date
- Jan-2013
- Publisher
- SPRINGER
- Keywords
- Dunaliella salina; Phosphoenolpyruvate carboxylase; Escherichia coli; Malate; Oxaloacetate
- Citation
- BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.36, no.1, pp.127 - 131
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOPROCESS AND BIOSYSTEMS ENGINEERING
- Volume
- 36
- Number
- 1
- Start Page
- 127
- End Page
- 131
- ISSN
- 1615-7591
- Abstract
- A new phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina is identified using homology analysis was conducted using PEPC gene of Chlamydomonas reinhardtii and Arabidopsis thaliana. Recombinant E. coli SGJS115 with increased production of malate and oxaloacetate was developed by introducing codon-optimized phosphoenolpyruvate carboxylase2 (OPDSPEPC2) gene of Dunaliella salina. E. coli SGJS115 yielded a 9.9 % increase in malate production. In addition, E. coli SGJS115 exhibited two times increase in the yield of oxaloacetate over the E. coli SGJS114 having identified PEPC2 gene obtained from Dunaliella salina.
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Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
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Related Researcher
- Jin, Eon Seon
- COLLEGE OF NATURAL SCIENCES (DEPARTMENT OF LIFE SCIENCE)