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Comparison of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay with conventional methods for detection of IMP-6, VIM-2, NDM-1, SIM-1, KPC-1, OXA-23, and OXA-51 carbapenemase-producing Acinetobacter spp., Pseudomonas aeruginosa, and Klebsiella pneumoniae
- Authors
- Lee, Wonmok; Chung, Hae-Sun; Lee, Yang soon; Yong, Dongeun; Jeong, Seok Hoon; Lee, Kyungwon; Chong, Yunsop
- Issue Date
- 2013
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- Acinetobacter spp; Carbapenemase; MALDI-TOF MS; OXA; SIM-1
- Citation
- Diagnostic Microbiology and Infectious Disease, v.77, no.3, pp.227 - 230
- Indexed
- SCIE
SCOPUS
- Journal Title
- Diagnostic Microbiology and Infectious Disease
- Volume
- 77
- Number
- 3
- Start Page
- 227
- End Page
- 230
- ISSN
- 0732-8893
- Abstract
- Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry assay was able to detect carbapenemase producers, including SIM-1 or OXA-51, within 4 hours using 20 mu L of 0.5 g/L ertapenem solution as a substrate. This assay is more rapid and accurate than the modified Hodge test and 3-dimensional extract bioassay. Hence, it can be used an alternative test to identify carbapenemase-mediated carbapenem resistance in Gram-negative bacteria. (C) 2013 Elsevier Inc. All rights reserved.
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Related Researcher
- Lee, Yang soon
- COLLEGE OF MEDICINE (DEPARTMENT OF LABORATORY MEDICINE)