Identification of a novel cell-penetrating peptide from human phosphatidate phosphatase LPIN3
DC Field | Value | Language |
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dc.contributor.author | Lim, Sangho | - |
dc.contributor.author | Kim, Won-ju | - |
dc.contributor.author | Kim, Yeon-ho | - |
dc.contributor.author | Choi, Je-Min | - |
dc.date.accessioned | 2022-07-16T12:33:28Z | - |
dc.date.available | 2022-07-16T12:33:28Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164068 | - |
dc.description.abstract | Biomolecules such as proteins, DNA, and RNA are macromolecules and can not cross the cell membrane. However, cell-penetrating peptide (CPP) has been shown to deliver therapeutic biomolecules successfully into cells. The various and widely used CPPs including TAT, VP22, and Antp are mostly non-human originated CPPs, and are limited by their potential toxicity and immunogenicity. We report here on a newly identified novel cell-penetrating sequence (LPIN; RRKRRRRRK) from the nuclear localization sequence (NLS) of human nuclear phosphatase, LPIN3. LPIN-EGFP recombinant protein was concentration- and time-dependently delivered into cells and localized to the nucleus as well as the cytoplasm. It penetrated the cell membrane by lipid raft-mediated endocytosis by binding to heparan sulfate proteoglycan. LPIN-EGFP was successfully delivered into primary mouse splenocytes in vitro and it could be delivered into various tissues including liver, kidney, and intestine in mice after intra-peritoneal injection. This research suggests that LPIN-CPP could be used in a drug delivery system to deliver therapeutic biomolecules including peptides, proteins, DNA, and RNA and without the limitations of non-human originated CPPs such as TAT-CPP. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.title | Identification of a novel cell-penetrating peptide from human phosphatidate phosphatase LPIN3 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Je-Min | - |
dc.identifier.doi | 10.1007/s10059-012-0284-y | - |
dc.identifier.scopusid | 2-s2.0-84880060102 | - |
dc.identifier.wosid | 000312724600011 | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.34, no.6, pp.577 - 582 | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 577 | - |
dc.citation.endPage | 582 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001718080 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | ALLERGIC AIRWAY INFLAMMATION | - |
dc.subject.keywordPlus | PROTEIN TRANSDUCTION | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | CYTOPLASMIC DOMAIN | - |
dc.subject.keywordPlus | THERAPEUTICS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordAuthor | CPP | - |
dc.subject.keywordAuthor | drug delivery system | - |
dc.subject.keywordAuthor | LPIN | - |
dc.subject.keywordAuthor | PTD | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s10059-012-0284-y | - |
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