Identification of a novel cell-penetrating peptide from human phosphatidate phosphatase LPIN3
- Authors
- Lim, Sangho; Kim, Won-ju; Kim, Yeon-ho; Choi, Je-Min
- Issue Date
- Dec-2012
- Publisher
- KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
- Keywords
- CPP; drug delivery system; LPIN; PTD
- Citation
- MOLECULES AND CELLS, v.34, no.6, pp.577 - 582
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- MOLECULES AND CELLS
- Volume
- 34
- Number
- 6
- Start Page
- 577
- End Page
- 582
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164068
- DOI
- 10.1007/s10059-012-0284-y
- ISSN
- 1016-8478
- Abstract
- Biomolecules such as proteins, DNA, and RNA are macromolecules and can not cross the cell membrane. However, cell-penetrating peptide (CPP) has been shown to deliver therapeutic biomolecules successfully into cells. The various and widely used CPPs including TAT, VP22, and Antp are mostly non-human originated CPPs, and are limited by their potential toxicity and immunogenicity. We report here on a newly identified novel cell-penetrating sequence (LPIN; RRKRRRRRK) from the nuclear localization sequence (NLS) of human nuclear phosphatase, LPIN3. LPIN-EGFP recombinant protein was concentration- and time-dependently delivered into cells and localized to the nucleus as well as the cytoplasm. It penetrated the cell membrane by lipid raft-mediated endocytosis by binding to heparan sulfate proteoglycan. LPIN-EGFP was successfully delivered into primary mouse splenocytes in vitro and it could be delivered into various tissues including liver, kidney, and intestine in mice after intra-peritoneal injection. This research suggests that LPIN-CPP could be used in a drug delivery system to deliver therapeutic biomolecules including peptides, proteins, DNA, and RNA and without the limitations of non-human originated CPPs such as TAT-CPP.
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