CD24 regulates cell proliferation and transforming growth factor beta-induced epithelial to mesenchymal transition through modulation of integrin beta 1 stability
- Authors
- Lee, Kyung-min; Ju, Ji-hyun; Jang, Kibeom; Yang, Wonseok; Yi, Jae Youn; Noh, Dong Young; Shin, Incheol
- Issue Date
- Nov-2012
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- CD24; EMT; ERK; FAK; Integrin
- Citation
- CELLULAR SIGNALLING, v.24, no.11, pp.2132 - 2142
- Indexed
- SCIE
SCOPUS
- Journal Title
- CELLULAR SIGNALLING
- Volume
- 24
- Number
- 11
- Start Page
- 2132
- End Page
- 2142
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/164298
- DOI
- 10.1016/j.cellsig.2012.07.005
- ISSN
- 0898-6568
- Abstract
- To determine the role of CD24 in breast cancer cells, we knocked down CD24 in MCF-7 human breast cancer cells by retroviral delivery of shRNA. MCF-7 cells with knocked down CD24 (MCF-7 hCD24 shRNA) exhibited decreased cell proliferation and cell adhesion as compared to control MCF-7 mCD24 shRNA cells. Decreased proliferation of MCF-7 hCD24 shRNA cells resulted from the inhibition of cell cycle progression from G1 to S phase. The specific inhibition of MEK/ERK signaling by CD24 ablation might be responsible for the inhibition of cell proliferation. Phosphorylation of Src/FAK and TGF-beta 1 -mediated epithelial to mesenchymal transition was also down-regulated in MCF-7 hCD24 shRNA cells. Reduced Src/FAK activity was caused by a decrease in integrin beta 1 bound with CD24 and subsequent destabilization of integrin beta 1. Our results suggest that down-regulation of Raf/MEK/ERK signaling via Src/FAK may be dependent on integrin beta 1 function and that this mechanism is largely responsible for the CD24 ablation-induced decreases in cell proliferation and epithelial to mesenchymal transition.
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