Preparation of Biomimetic Hydrogels with Controlled Cell Adhesive Properties and Topographical Features for the Study of Muscle Cell Adhesion and Proliferation

Abstract

Synthetic substrates with defined chemical and structural characteristics may potentially be prepared to mimic the living ECM to regulate cell adhesion and growth. Hydrogels with cell-adhesive peptides (0.28 +/- 0.03 nmol peptide . cm(-2), TTA-R-0.5; and 0.91 +/- 0.12 nmol peptide . cm(-2), TTA-R-2.0) and/or micro-scaled topographical patterns (10, 25, and 80 mu m grooves) are prepared using enzymatic polymerization. The adherent morphology and proliferation of C2C12 skeletal myoblasts and human aortic smooth muscle cells (hAoSM) on the hydrogels are studied. The newly developed hydrogels may be useful in investigating the roles of cell adhesion and substrate surface properties in the communication of adherent cells with the ECM.