Loss of Mel-18 induces tumor angiogenesis through enhancing the activity and expression of HIF-1 alpha mediated by the PTEN/PI3K/Akt pathway
- Authors
- Park, J-H; Lee, J-Y; Shin, D-H; Jang, K-S; Kim, H-J; Kong, Gu
- Issue Date
- Nov-2011
- Publisher
- NATURE PUBLISHING GROUP
- Keywords
- Mel-18; polycomb group proteins; Akt; angiogenesis
- Citation
- ONCOGENE, v.30, no.45, pp.4578 - 4589
- Indexed
- SCIE
SCOPUS
- Journal Title
- ONCOGENE
- Volume
- 30
- Number
- 45
- Start Page
- 4578
- End Page
- 4589
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167259
- DOI
- 10.1038/onc.2011.174
- ISSN
- 0950-9232
- Abstract
- Mel-18 has been implicated in several processes in tumor progression, in which the Akt pathway is involved as an important key molecular event. However, the function of Mel-18 in human cancers has not been fully established yet. Here, we examined the effect of Mel-18 on tumor angiogenesis in human breast cancer, and found that Mel-18 was a novel regulator of HIF-1 alpha. Mel-18 negatively regulated the HIF-1 alpha expression and its target gene VEGF transcription during both normoxia and hypoxia. We demonstrated that Mel-18 regulated the HIF-1 alpha expression and activity via the PI3K/Akt pathway. Loss of Mel-18 downregulated Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression, consequently activating the PI3K/Akt/MDM2 pathway, and leading to an increase of HIF-1 alpha protein level. Mel-18 modulated the HIF-1 alpha transcriptional activity via regulating the cytoplasmic retention of FOXO3a, a downstream effector of Akt, and recruitment of HIF-1 alpha/CBP complex to the VEGF promoter. Furthermore, our data shows that Mel-18 blocked tumor angiogenesis both in vitro and in vivo. Mel-18 overexpression inhibited in vitro tube formation in human umbilical endothelial cells (HUVECs). Xenografts in NOD/SCID mice derived from stably Mel-18 knocked down MCF7 human breast cancer cells showed increased tumor volume, microvessel density, and phospho-Akt and HIF-1a expression levels. In conclusion, our findings provide that Mel-18 is a novel regulator of tumor angiogenesis through regulating HIF-1 alpha and its target VEGF expressions mediated by the PTEN/PI3K/Akt pathway, suggesting a new tumor-suppressive role of Mel-18 in human breast cancer.
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