CD24 enhances DNA damage-induced apoptosis by modulating NF-kappa B signaling in CD44-expressing breast cancer cellsopen access
- Authors
- Ju, Ji-hyun; Jang, Kibeom; Lee, Kyung-min; Kim, Minsoon; Kim, Jongbin; Yi, Jae Youn; Noh, Dong-Young; Shin, Incheol
- Issue Date
- Oct-2011
- Publisher
- OXFORD UNIV PRESS
- Citation
- CARCINOGENESIS, v.32, no.10, pp.1474 - 1483
- Indexed
- SCIE
SCOPUS
- Journal Title
- CARCINOGENESIS
- Volume
- 32
- Number
- 10
- Start Page
- 1474
- End Page
- 1483
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167480
- DOI
- 10.1093/carcin/bgr173
- ISSN
- 0143-3334
- Abstract
- Cluster of differentiation 24 (CD24) is a small glycosylphosphatidylinositol-linked cell surface molecule that is expressed in a variety of human carcinomas, including breast cancer. To determine the role of CD24 in breast cancer cells, we expressed CD24 in CD24-negative/low and cluster of differentiation 44 (CD44)-positive MDA-MB-231 metastatic breast cancer cells. Forced expression of CD24 resulted in a decrease in c-Raf/mitogen-activated protein/extracellular signal-regulated kinase kinase (MEK)/mitogen-activated protein kinase signaling and reduced cell proliferation. Apoptosis induced by DNA damage was greatly enhanced in MDA-MB-231 CD24 cells as compared with MDA-MB-231 vec cells. CD24 expression efficiently attenuated DNA damage-induced nuclear factor-kappaB (NF-kappa B) signaling in MDA-MB-231 cells. However, in CD24-positive and CD44-negative/low MCF-7 cells, knockdown of CD24 did not significantly affect DNA damage-induced apoptosis nor NF-kappa B signaling. Silencing of CD24 in CD24/CD44-double-positive MDA-MB-468 cells partially rescued DNA damage-induced apoptosis. Transient transfection studies with 293T cells also revealed that CD24 attenuated cell viability and NF-kappa B signaling only when CD44 was cotransfected. These data indicate that CD24 expression potentiated DNA-induced apoptosis by suppressing antiapoptotic NF-kappa B signaling in CD44-expressing cells.
- Files in This Item
-
- Appears in
Collections - 서울 자연과학대학 > 서울 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167480)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.