Transcriptomic Analysis of Haematococcus lacustris during Astaxanthin Accumulation under High Irradiance and Nutrient Starvation
- Authors
- Kim, Dong-Keon; Hong, Seong-Joo; Bae, Jae-Han; Yim, Narae; Jin, EonSeon; Lee, Choul-Gyun
- Issue Date
- Aug-2011
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- Haematococcus lacustris; astaxanthin; cDNA microarray; transcriptomics; high irradiance; nutrient starvation
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.16, no.4, pp.698 - 705
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 16
- Number
- 4
- Start Page
- 698
- End Page
- 705
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/167869
- DOI
- 10.1007/s12257-011-0081-z
- ISSN
- 1226-8372
- Abstract
- In this study, a cDNA microarray was developed from over 60,000 mRNA readings to analyze the expression profiles of transcriptomes of Haematococcus lacustris under astaxanthin-inducing culture conditions, high irradiance and nitrate starvation. Among 20,033 genes on the cDNA microarray, 2,675 genes exhibited a twofold or greater difference in expression. Of these, 1,333 genes were up-regulated and 1,342 genes were down-regulated. A significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were observed under astaxanthin inducing conditions (forming red cyst cells). On the other hand, respiration-related genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells under stress conditions. These results enabled a better understanding of the cell responses during stress induction of H. lacustris such as photosynthesis, respiration, and some biopathways.
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