Carbapenem-non-susceptible Acinetobacter baumannii of sequence type 92 or its single-locus variants with a G428T substitution in zone 2 of the rpoB gene
- Authors
- Lee, Yang soon; Lee, Jinhwa; Jeong, Seok Hoon; Lee, Jonghan; Bae, Il Kwon; Lee, Kyungwon
- Issue Date
- 2011
- Publisher
- OXFORD UNIV PRESS
- Keywords
- Clonal complex 92; European clone II; Multilocus sequence typing
- Citation
- JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, v.66, pp.66 - 72
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
- Volume
- 66
- Start Page
- 66
- End Page
- 72
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/169337
- DOI
- 10.1093/jac/dkq402
- ISSN
- 0305-7453
- Abstract
- Objectives
To investigate the epidemiological traits of carbapenem-non-susceptible Acinetobacter baumannii (CNSAB) and the usefulness of phylogenetic grouping based on partial rpoB gene sequencing in defining the epidemiological traits of CNSAB.
Methods
A total of 547 non-duplicate clinical isolates of Acinetobacter spp. were collected from 19 hospitals in Korea in 2008. Detection of genes encoding OXA carbapenemases and metallo-β-lactamases was performed by PCR. The epidemiological relationships of the isolates were investigated by multilocus sequence typing and repetitive-sequence-based PCR. The 450 bp sequence (zone 2) of the rpoB gene was amplified and sequenced.
Results
Molecular characterization of the 272 CNSAB isolates identified five sequence types (STs): ST92, ST75, ST137, ST138 and ST69. The first four of these STs were clustered into clonal complex (CC) 92, sharing alleles at six of seven housekeeping gene loci; ST69 shared alleles at five of seven loci. CNSAB of CC92 carried the blaₒₓₐ₋₂₃ gene (n = 169), the blaₒₓₐ₋₅₁-like gene preceded by ISAba1 (n = 89) or both (n = 14). Notably, all CNSAB isolates carried a G428T substitution in zone 2 of the rpoB gene.
Conclusions
CNSAB isolates of CC92 with the G428T substitution in zone 2 of the rpoB gene are disseminated nationwide in Korea. A. baumannii with the single nucleotide substitution may be more likely to acquire carbapenem resistance than are other isolates.
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