Development of Three-Stage Bioaerosol Sampler for Size-Selective Sampling
- Authors
- Lim, Jun-Hyung; Nam, Sang Hwan; Kim, Jongwoo; Kim, Nam Hoon; Park, Gun-Soo; Maeng, Jin-Soo; Yook, Se-Jin
- Issue Date
- Jul-2022
- Publisher
- ASME
- Keywords
- bioaerosol; collection efficiency; cyclone separator; virtual impactor; BioSampler
- Citation
- JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, v.144, no.7, pp.1 - 7
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
- Volume
- 144
- Number
- 7
- Start Page
- 1
- End Page
- 7
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/170068
- DOI
- 10.1115/1.4053504
- ISSN
- 0148-0731
- Abstract
- In this study, a three-stage bio-aerosol sampler with a sampling flow rate of 170 L/min was designed and fabricated for sampling the bio-aerosols released during human breathing and coughing, and its performance was evaluated. The sampler was constructed using a cyclone separator with a cutoff size of 2.5 μm as a preseparator, a multinozzle virtual impactor with a cutoff size of 0.34 μm as an aerosol concentrator, and a Bio-Sampler, which is a commercial product, for collecting bio-aerosols in a collection fluid. The collection efficiency of the sampler was evaluated through simulations and experiments. Only particles with sizes of 0.1-4 μm were selectively collected in the collection fluid. Bacteriophage bio-aerosols were sampled using the developed sampler and ACD-200 Bobcat sampler, which is a commercial product. The amounts of collected bacteriophages were compared using the polymerase chain reaction (PCR) technique. The sampling performance of the developed sampler was similar to that of the ACD-200 Bobcat sampler. Moreover, the developed sampler showed its ability to sample bio-aerosols of a specific size range and collect them directly in a collection fluid for the PCR analysis. Therefore, the developed sampler is expected to be useful for indoor environmental monitoring by effectively sampling the bio-aerosols released indoors during human breathing and coughing.
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