In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae Won | - |
dc.contributor.author | Kim, Hyun Seung | - |
dc.contributor.author | Yon, Soo-Jeong | - |
dc.contributor.author | Matsumoto, Takuya | - |
dc.contributor.author | Lee, Sang-Kyung | - |
dc.contributor.author | Lee, Kuen Yong | - |
dc.date.accessioned | 2022-07-19T04:55:52Z | - |
dc.date.available | 2022-07-19T04:55:52Z | - |
dc.date.created | 2022-06-03 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 0141-8130 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/170098 | - |
dc.description.abstract | Stem cells exist and maintain their quiescence and pluripotency in stem cell niche. Here, we hypothesized that regulation of cell-cell interactions using a polymeric scaffold as synthetic extracellular matrix (ECM) could be critical in creating a hematopoietic stem cell (HSC) niche in vitro. Angiopoietin-1 (Ang1) binds to the tyrosine kinase receptor (Tie2), and regulation of the Tie2/Ang1 interaction is important in maintaining the quiescence of HSCs in vivo. Alginate hydrogel was thus modified with Ang1 as a synthetic ECM to mimic the HSC niche. Long-term HSCs (CD34−, CD135−, and CD150+) were isolated from mouse femurs and cultured on Ang1-modified alginate hydrogel. The percentage of LT-HSCs in G0 phase was 46.8 ± 1.8%, which was comparable to that of LT-HSCs co-cultured with osteoblasts (46.8 ± 2.1%). Ang1-coupled alginate gels were useful to provide a niche for HSC quiescence without a co-culture system. Polymeric scaffolds containing biomimetic and cell-instructive characteristics for stem cell phenotype regulation might help create HSC niches in vitro and be useful to engineer tissues and transplant stem cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier B.V. | - |
dc.title | In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Sang-Kyung | - |
dc.contributor.affiliatedAuthor | Lee, Kuen Yong | - |
dc.identifier.doi | 10.1016/j.ijbiomac.2022.04.163 | - |
dc.identifier.scopusid | 2-s2.0-85129114833 | - |
dc.identifier.wosid | 000805954500005 | - |
dc.identifier.bibliographicCitation | International Journal of Biological Macromolecules, v.209, pp.1893 - 1899 | - |
dc.relation.isPartOf | International Journal of Biological Macromolecules | - |
dc.citation.title | International Journal of Biological Macromolecules | - |
dc.citation.volume | 209 | - |
dc.citation.startPage | 1893 | - |
dc.citation.endPage | 1899 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | PROGENITOR CELLS | - |
dc.subject.keywordPlus | BLOOD STEM | - |
dc.subject.keywordPlus | EXPANSION | - |
dc.subject.keywordPlus | QUIESCENCE | - |
dc.subject.keywordPlus | TRANSPLANTATION | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | CYCLE | - |
dc.subject.keywordPlus | MAINTENANCE | - |
dc.subject.keywordPlus | NANOFIBERS | - |
dc.subject.keywordAuthor | Hematopoietic stem cell | - |
dc.subject.keywordAuthor | Synthetic niche | - |
dc.subject.keywordAuthor | Alginate hydrogel | - |
dc.subject.keywordAuthor | Angiopoietin-1 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0141813022008777?via%3Dihub | - |
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