In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel
- Authors
- Lee, Jae Won; Kim, Hyun Seung; Yon, Soo-Jeong; Matsumoto, Takuya; Lee, Sang-Kyung; Lee, Kuen Yong
- Issue Date
- Jun-2022
- Publisher
- Elsevier B.V.
- Keywords
- Hematopoietic stem cell; Synthetic niche; Alginate hydrogel; Angiopoietin-1
- Citation
- International Journal of Biological Macromolecules, v.209, pp.1893 - 1899
- Indexed
- SCIE
SCOPUS
- Journal Title
- International Journal of Biological Macromolecules
- Volume
- 209
- Start Page
- 1893
- End Page
- 1899
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/170098
- DOI
- 10.1016/j.ijbiomac.2022.04.163
- ISSN
- 0141-8130
- Abstract
- Stem cells exist and maintain their quiescence and pluripotency in stem cell niche. Here, we hypothesized that regulation of cell-cell interactions using a polymeric scaffold as synthetic extracellular matrix (ECM) could be critical in creating a hematopoietic stem cell (HSC) niche in vitro. Angiopoietin-1 (Ang1) binds to the tyrosine kinase receptor (Tie2), and regulation of the Tie2/Ang1 interaction is important in maintaining the quiescence of HSCs in vivo. Alginate hydrogel was thus modified with Ang1 as a synthetic ECM to mimic the HSC niche. Long-term HSCs (CD34−, CD135−, and CD150+) were isolated from mouse femurs and cultured on Ang1-modified alginate hydrogel. The percentage of LT-HSCs in G0 phase was 46.8 ± 1.8%, which was comparable to that of LT-HSCs co-cultured with osteoblasts (46.8 ± 2.1%). Ang1-coupled alginate gels were useful to provide a niche for HSC quiescence without a co-culture system. Polymeric scaffolds containing biomimetic and cell-instructive characteristics for stem cell phenotype regulation might help create HSC niches in vitro and be useful to engineer tissues and transplant stem cells.
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