Organ-Specific Differentiation of Human Adipose-Derived Stem Cells in Various Organs of Xenotransplanted Rats: A Pilot Study
DC Field | Value | Language |
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dc.contributor.author | Park, Jung Ho | - |
dc.contributor.author | Choi, Yeon Ju | - |
dc.contributor.author | Kang, So Young | - |
dc.contributor.author | Ju, Hyunjeong | - |
dc.contributor.author | Min, Kyueng-Whan | - |
dc.contributor.author | Kim, Nan Young | - |
dc.contributor.author | Park, Ha Young | - |
dc.contributor.author | Kim, Eun Soo | - |
dc.contributor.author | Kwon, Mi Jung | - |
dc.contributor.author | Suh, Yong Joon | - |
dc.date.accessioned | 2022-10-25T07:43:40Z | - |
dc.date.available | 2022-10-25T07:43:40Z | - |
dc.date.created | 2022-10-06 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 0024-3019 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/172585 | - |
dc.description.abstract | Adipose-derived stem cells (ADSCs) are potential therapeutics considering their self-renewal capacity and ability to differentiate into all somatic cell types in vitro. The ideal ADSC-based therapy is a direct injection into the relevant organs. The objective of this study was to investigate the viability and safety of intra-organ human ADSC (h-ADSC) xenotransplants in vivo. Subcutaneous adipose tissue from the abdominal area of 10 patients was sampled. h-ADSCs were isolated from adipose tissue samples and identified using immunofluorescence antibodies. Multi-differentiation potential assays for adipocytes, osteocytes, and chondrocytes were performed. Cultured h-ADSCs at passage 4 were transplanted into multiple organs of 17 rats, including the skin, subcutaneous layer, liver, kidney, pancreas, and spleen. The h-ADSC-injected organs excised after 100 days were examined, and the survival of h-ADSCs was measured by quantitative real-time polymerase chain reaction (qRT-PCR) using specific human and rat target genes. h-ADSCs confirmed by stem cell phenotyping were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages in vitro. All rats were healthy and exhibited no side effects during the study; the transplanted h-ADSCs did not cause inflammation and were indiscernible from the native organ cells. The presence of transplanted h-ADSCs was confirmed using qRT-PCR. However, the engrafted survival rates varied as follows: subcutaneous fat (70.6%), followed by the liver (52.9%), pancreas (50.0%), kidney (29.4%), skin (29.4%), and spleen (12.5%). h-ADSCs were successfully transplanted into a rat model, with different survival rates depending on the organ. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | Organ-Specific Differentiation of Human Adipose-Derived Stem Cells in Various Organs of Xenotransplanted Rats: A Pilot Study | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Min, Kyueng-Whan | - |
dc.identifier.doi | 10.3390/life12081116 | - |
dc.identifier.scopusid | 2-s2.0-85137373422 | - |
dc.identifier.wosid | 000845476800001 | - |
dc.identifier.bibliographicCitation | LIFE-BASEL, v.12, no.8, pp.1 - 19 | - |
dc.relation.isPartOf | LIFE-BASEL | - |
dc.citation.title | LIFE-BASEL | - |
dc.citation.volume | 12 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 19 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | STROMAL CELLS | - |
dc.subject.keywordPlus | INTERNATIONAL-SOCIETY | - |
dc.subject.keywordPlus | LIVER FIBROSIS | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | RECONSTRUCTION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | adult stem cell | - |
dc.subject.keywordAuthor | adipose-derived stem cell | - |
dc.subject.keywordAuthor | direct intra-organ xenotransplantation | - |
dc.subject.keywordAuthor | differentiation | - |
dc.subject.keywordAuthor | rats | - |
dc.identifier.url | https://www.mdpi.com/2075-1729/12/8/1116 | - |
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