Organ-Specific Differentiation of Human Adipose-Derived Stem Cells in Various Organs of Xenotransplanted Rats: A Pilot Studyopen access
- Authors
- Park, Jung Ho; Choi, Yeon Ju; Kang, So Young; Ju, Hyunjeong; Min, Kyueng-Whan; Kim, Nan Young; Park, Ha Young; Kim, Eun Soo; Kwon, Mi Jung; Suh, Yong Joon
- Issue Date
- Aug-2022
- Publisher
- MDPI
- Keywords
- adult stem cell; adipose-derived stem cell; direct intra-organ xenotransplantation; differentiation; rats
- Citation
- LIFE-BASEL, v.12, no.8, pp.1 - 19
- Indexed
- SCIE
SCOPUS
- Journal Title
- LIFE-BASEL
- Volume
- 12
- Number
- 8
- Start Page
- 1
- End Page
- 19
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/172585
- DOI
- 10.3390/life12081116
- ISSN
- 0024-3019
- Abstract
- Adipose-derived stem cells (ADSCs) are potential therapeutics considering their self-renewal capacity and ability to differentiate into all somatic cell types in vitro. The ideal ADSC-based therapy is a direct injection into the relevant organs. The objective of this study was to investigate the viability and safety of intra-organ human ADSC (h-ADSC) xenotransplants in vivo. Subcutaneous adipose tissue from the abdominal area of 10 patients was sampled. h-ADSCs were isolated from adipose tissue samples and identified using immunofluorescence antibodies. Multi-differentiation potential assays for adipocytes, osteocytes, and chondrocytes were performed. Cultured h-ADSCs at passage 4 were transplanted into multiple organs of 17 rats, including the skin, subcutaneous layer, liver, kidney, pancreas, and spleen. The h-ADSC-injected organs excised after 100 days were examined, and the survival of h-ADSCs was measured by quantitative real-time polymerase chain reaction (qRT-PCR) using specific human and rat target genes. h-ADSCs confirmed by stem cell phenotyping were induced to differentiate into adipogenic, osteogenic, and chondrogenic lineages in vitro. All rats were healthy and exhibited no side effects during the study; the transplanted h-ADSCs did not cause inflammation and were indiscernible from the native organ cells. The presence of transplanted h-ADSCs was confirmed using qRT-PCR. However, the engrafted survival rates varied as follows: subcutaneous fat (70.6%), followed by the liver (52.9%), pancreas (50.0%), kidney (29.4%), skin (29.4%), and spleen (12.5%). h-ADSCs were successfully transplanted into a rat model, with different survival rates depending on the organ.
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