Proteomic analysis of oxidative stress-induced neuronal cell death by using two-dimensional fluorescence difference gel electrophoresisopen access
- Authors
- Kim, Eun Young; Yoon, Tae-Sung; Bahn, Young Jae; Jeong, Dae Gwin; Park, Mi Rim; Chung, Sang J.; Park, Sung Goo; Park, Byoung Chul; Lee, Sang Chul; Ryu, Seong Eon; Bae, Kwang-Hee
- Issue Date
- Dec-2010
- Publisher
- SPANDIDOS PUBL LTD
- Keywords
- two-dimensional fluorescence difference gel electrophoresis; HT22; oxidative stress; proteomics; reactive oxygen species
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, v.26, no.6, pp.829 - 835
- Indexed
- SCIE
SCOPUS
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
- Volume
- 26
- Number
- 6
- Start Page
- 829
- End Page
- 835
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/173376
- DOI
- 10.3892/ijmm_00000531
- ISSN
- 1107-3756
- Abstract
- Oxidative stress has been implicated in a number of neurological disorders, including cerebral ischemia and neurodegenerative diseases. Comprehensive proteomic studies were carried out using an immortalized mouse hippocampal cell line, HT22, exhibiting oxidative stress-mediated cell death upon glutamate treatment. Two-dimensional fluorescence difference gel electrophoresis (2D DIGE) of subcellular organelle fractions revealed that significant numbers of proteins showed quantitative changes during HT22 cell death, among which a total of 51 proteins were identified by mass spectrometry. The identified proteins indicate that HT22 cell death occurs through perturbations in mitochondrial function, changes in translational elongation machinery, and translocation of proteins across subcellular organelles. This list of proteins may shed light on oxidative stress-mediated neuronal cell death.
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