Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditionsopen access
- Authors
- Lim, J. J.; Sung, S. -Y.; Kim, H. J.; Song, S. -H.; Hong, J. Y.; Yoon, T. K.; Kim, J. K.; Kim, K. -S.; Lee, D. R.
- Issue Date
- Aug-2010
- Publisher
- WILEY-BLACKWELL
- Citation
- CELL PROLIFERATION, v.43, no.4, pp.405 - 417
- Indexed
- SCIE
SCOPUS
- Journal Title
- CELL PROLIFERATION
- Volume
- 43
- Number
- 4
- Start Page
- 405
- End Page
- 417
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174375
- DOI
- 10.1111/j.1365-2184.2010.00691.x
- ISSN
- 0960-7722
- Abstract
- Objectives: The aim of the present study was to improve efficiency of isolation and to optimize proliferative potential of human spermatogonial stem cells (SSCs) obtained from obstructive azoospermic (OA) and non-obstructive azoospermic (NOA) patients, and further, to characterize these cells for potential use in infertility treatment or study of reproductive biology. Materials and methods: We have applied a cell-sorting method, using collagen and magnetic activated cell separation to overcome obstacles, developing a collection system, and simple long-term proliferation system, that yields large numbers of high-purity SSCs from obstructive OA and NOA patients. Results: SSCs derived from OA and NOA patients proliferated and maintained their characteristics for more than 12 passages (> 6 months) in vitro. Moreover, the population of cells positive for the SSC-specific markers GFR alpha-1 and integrin alpha 6, increased to more than 80% at passage 8. Conclusion: These finding may support the idea that in vitro propagation of SSCs could be a useful tool for infertility treatment and study of reproductive biology.
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