Synthesis and Characterization of Dexamethasone-Conjugated Linear Polyethylenimine as a Gene Carrier
- Authors
- Kim, Hyunjung; Bae, Yun Mi; Kim, Hyun Ah; Hyun, Hyesun; Yu, Gwang Sig; Choi, Joon Sig; Lee, Minhyung
- Issue Date
- Jun-2010
- Publisher
- WILEY
- Keywords
- BRANCHED POLYETHYLENIMINE (BPEI); DEXAMETHASONE; GENE DELIVERY; LINEAR POLYETHYLENIMINE (PEI); TRANSFECTION
- Citation
- JOURNAL OF CELLULAR BIOCHEMISTRY, v.110, no.3, pp.743 - 751
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF CELLULAR BIOCHEMISTRY
- Volume
- 110
- Number
- 3
- Start Page
- 743
- End Page
- 751
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/174879
- DOI
- 10.1002/jcb.22587
- ISSN
- 0730-2312
- Abstract
- Linear polyethylenimine (25 kDa, LPEI25k) has been shown to be an effective non-viral gene carrier with higher transfection and lower toxicity than branched polyethylenimine (BPEI) of comparable molecular weight. In this study, dexamethasone was conjugated to LPE125k to improve the efficiency of gene delivery. Dexamethasone is a synthetic glucocorticoid receptor ligand. Dexamethasone-conjugated LPEI25k (LPEI-Dexa) was evaluated as a gene carrier in various cells. Gel retardation assays showed that LPEI-Dexa completely retarded plasmid DNA (pDNA) at a 0.75:1 weight ratio (LPEI/pDNA). LPEI-Dexa had the highest transfection efficiency at a 2:1 weight ratio (LPEI-Dexa/DNA). At this ratio, the size of the LPEI-Dexa/pDNA complex was approximately 125 nm and the zeta potential was 35 mV. LPEI-Dexa had higher transfection efficiency than LPEI and Lipofectamine 2000. In addition, the cytotoxicity of LPEI-Dexa was much lower than that of BPEI (25 kDa, BPEI25k). In conclusion, LPEI-Dexa has a high transfection efficiency and low toxicity and can therefore be used for non-viral gene delivery.
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