Tempol or candesartan prevents high-fat diet-induced hypertension and renal damage in spontaneously hypertensive ratsopen access
- Authors
- Chung, Sungjin; Park, Cheol Whee; Shin, Seok Joon; Lim, Ji Hee; Chung, Hyun Wha; Youn, Dong-Ye; Kim, Hyung Wook; Kim, Byung Soo; Lee, Jeong-Hwa; Kim, Gheun-Ho; Chang, Yoon Sik
- Issue Date
- Feb-2010
- Publisher
- OXFORD UNIV PRESS
- Keywords
- hypertension; lipotoxicity; obesity; oxidative stress; renin-angiotensin system
- Citation
- NEPHROLOGY DIALYSIS TRANSPLANTATION, v.25, no.2, pp.389 - 399
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEPHROLOGY DIALYSIS TRANSPLANTATION
- Volume
- 25
- Number
- 2
- Start Page
- 389
- End Page
- 399
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/175481
- DOI
- 10.1093/ndt/gfp472
- ISSN
- 0931-0509
- Abstract
- Methods. Male SHR and Wistar-Kyoto rats (WKY) were divided into eight groups: normal-fat diet-fed WKY (WKY-NF), high-fat diet-fed WKY (WKY-HF), high-fat diet-fed tempol-treated WKY (WKY-HF/T), high-fat diet-fed candesartan-treated WKY (WKY-HF/C), normal-fat diet-fed SHR (SHR-NF), high-fat diet-fed SHR (SHR-HF), high-fat diet-fed tempol-treated SHR (SHR-HF/T) and high-fat diet-fed candesartan-treated SHR (SHR-HF/C). After 12 weeks of treatment, haemodynamic measurements and histological assessment of the kidney were performed. Results. At the end of week 12, the high-fat fed SHR gained more body weight, their systolic blood pressure was further elevated and glucose intolerance induced. There was no significant difference in the insulin resistance index, serum lipid profile, plasma renin activity and serum aldosterone levels according to diet. However, the high-fat diet resulted in increases in immunohistochemical stains of renin and angiotensin II in the kidney. The real-time PCR also demonstrated significant increases in mRNA levels of renin, angiotensinogen and angiotensin-converting enzyme in the kidney, reflecting enhanced activation of the intrarenal RAS, which findings were also shown by Western blot analysis for renin and angiotensin II type 1 receptor. The expression of ED-1, osteopontin and TGF-beta 1 in the renal cortex were prominently enhanced in the SHR-HF group with the increased intrarenal lipid concentrations and oxidative stress. Administration of tempol or candesartan in the high-fat diet-induced SHR inhibited the elevation of the systolic blood pressure, intrarenal lipid concentrations, oxidative stress and the degree of renal inflammation to the levels of, or more than, the SHR-NF with no differences in the body weight and periepididymal fat weight, compared to those in the SHR-HF group without such treatment. Conclusions. Our study suggests that a high-fat diet induces fatty kidneys, aggravation of blood pressure and renal inflammation in the SHR. Blockade of oxidative stress by tempol or of RAS by candesartan ameliorates the increase in blood pressure and renal inflammation and improves intrarenal lipid accumulation. Therefore, antioxidants or angiotensin receptor blockers can prevent diet-induced hypertension and renal inflammation in hypertensive rats.
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