DHP-Derivative and Low Oxygen Tension Effectively Induces Human Adipose Stromal Cell Reprogrammingopen access
- Authors
- Jee, Min Ki; Kim, Ji Hoon; Han, Yong Man; Jung, Sung Jun; Kang, Kyung Sun; Kim, Dong Wook; Kang, Soo Kyung
- Issue Date
- Feb-2010
- Publisher
- PUBLIC LIBRARY SCIENCE
- Citation
- PLOS ONE, v.5, no.2, pp.1 - 14
- Indexed
- SCIE
SCOPUS
- Journal Title
- PLOS ONE
- Volume
- 5
- Number
- 2
- Start Page
- 1
- End Page
- 14
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/175515
- DOI
- 10.1371/journal.pone.0009026
- ISSN
- 1932-6203
- Abstract
- Background and Methods: In this study, we utilized a combination of low oxygen tension and a novel anti-oxidant, 4-(3,4-dihydroxy- phenyl)-derivative (DHP-d) to directly induce adipose tissue stromal cells (ATSC) to de-differentiate into more primitive stem cells. De-differentiated ATSCs was overexpress stemness genes, Rex-1, Oct-4, Sox-2, and Nanog. Additionally, demethylation of the regulatory regions of Rex-1, stemnesses, and HIF1 alpha and scavenging of reactive oxygen species were finally resulted in an improved stem cell behavior of de-differentiate ATSC (de-ATSC). Proliferation activity of ATSCs after dedifferentiation was induced by REX1, Oct4, and JAK/STAT3 directly or indirectly. De-ATSCs showed increased migration activity that mediated by P38/JUNK and ERK phosphorylation. Moreover, regenerative efficacy of de-ATSC engrafted spinal cord-injured rats and chemical-induced diabetes animals were significantly restored their functions. Conclusions/Significance: Our stem cell remodeling system may provide a good model which would provide insight into the molecular mechanisms underlying ATSC proliferation and transdifferentiation. Also, these multipotent stem cells can be harvested may provide us with a valuable reservoir of primitive and autologous stem cells for use in a broad spectrum of regenerative cell-based disease therapy.
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