Square wave voltammetric detection of Anthrax utilizing a peptide for selective recognition of a protein biomarker
DC Field | Value | Language |
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dc.contributor.author | Huan, Tran Ngoc | - |
dc.contributor.author | Ha, Vu Thi Thu | - |
dc.contributor.author | Hung, Le Quoc | - |
dc.contributor.author | Yoon, Moon-Young | - |
dc.contributor.author | Han, Sung-Hwan | - |
dc.contributor.author | Chung, Hoeil | - |
dc.date.accessioned | 2022-12-20T20:40:27Z | - |
dc.date.available | 2022-12-20T20:40:27Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-10 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176137 | - |
dc.description.abstract | A short chain peptide (26mer) has been successfully utilized for the selective electrochemical detection of the protein biomarker, protective antigen (PA), for the diagnosis of Anthrax. The major motivation of using a peptide instead of an antibody for the development of a biosensor is that there are advantages associated with the smaller size, better biological stability and easy synthesizability of a peptide. PA-selective peptide was synthesized and conjugated on a binding layer previously immobilized onto gold electrode. The same sensing scheme using a conventional antibody instead of a peptide was also tested for the purpose of comparison. Since the size of the peptide is approximately 1-2 orders of magnitude smaller than that of the antibody, a more populated immobilization of peptide on the sensing layer is possible and will eventually lead to improved sensitivity for PA detection. The selectivity of the peptide-based sensor was evaluated by observing the reduction peaks of samples containing PA with different concentrations of bovine serum albumin (BSA). The resulting responses were interference-free from BSA. The results of this study demonstrate the strong analytical potential of a peptide-based biosensor for diverse applications, especially in disease diagnosis where detection of a specific protein biomarker is particularly demanding. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Square wave voltammetric detection of Anthrax utilizing a peptide for selective recognition of a protein biomarker | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Hoeil | - |
dc.identifier.doi | 10.1016/j.bios.2009.08.002 | - |
dc.identifier.scopusid | 2-s2.0-69949117618 | - |
dc.identifier.wosid | 000270764300033 | - |
dc.identifier.bibliographicCitation | BIOSENSORS & BIOELECTRONICS, v.25, no.2, pp.469 - 474 | - |
dc.relation.isPartOf | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.title | BIOSENSORS & BIOELECTRONICS | - |
dc.citation.volume | 25 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 469 | - |
dc.citation.endPage | 474 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | BACILLUS-ANTHRACIS | - |
dc.subject.keywordPlus | PROTECTIVE ANTIGEN | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | LETHAL FACTOR | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | SPORES | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | Anthrax | - |
dc.subject.keywordAuthor | Protective antigen | - |
dc.subject.keywordAuthor | Peptide immobilization | - |
dc.subject.keywordAuthor | Square wave voltammetry | - |
dc.subject.keywordAuthor | Biosensor | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0956566309004230?via%3Dihub | - |
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