Square wave voltammetric detection of Anthrax utilizing a peptide for selective recognition of a protein biomarker
- Authors
- Huan, Tran Ngoc; Ha, Vu Thi Thu; Hung, Le Quoc; Yoon, Moon-Young; Han, Sung-Hwan; Chung, Hoeil
- Issue Date
- Oct-2009
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- Anthrax; Protective antigen; Peptide immobilization; Square wave voltammetry; Biosensor
- Citation
- BIOSENSORS & BIOELECTRONICS, v.25, no.2, pp.469 - 474
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 25
- Number
- 2
- Start Page
- 469
- End Page
- 474
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176137
- DOI
- 10.1016/j.bios.2009.08.002
- ISSN
- 0956-5663
- Abstract
- A short chain peptide (26mer) has been successfully utilized for the selective electrochemical detection of the protein biomarker, protective antigen (PA), for the diagnosis of Anthrax. The major motivation of using a peptide instead of an antibody for the development of a biosensor is that there are advantages associated with the smaller size, better biological stability and easy synthesizability of a peptide. PA-selective peptide was synthesized and conjugated on a binding layer previously immobilized onto gold electrode. The same sensing scheme using a conventional antibody instead of a peptide was also tested for the purpose of comparison. Since the size of the peptide is approximately 1-2 orders of magnitude smaller than that of the antibody, a more populated immobilization of peptide on the sensing layer is possible and will eventually lead to improved sensitivity for PA detection. The selectivity of the peptide-based sensor was evaluated by observing the reduction peaks of samples containing PA with different concentrations of bovine serum albumin (BSA). The resulting responses were interference-free from BSA. The results of this study demonstrate the strong analytical potential of a peptide-based biosensor for diverse applications, especially in disease diagnosis where detection of a specific protein biomarker is particularly demanding.
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