Proinflammatory Cytokine and Nitric Oxide Production by Human Macrophages Stimulated with Trichomonas vaginalis
- Authors
- Han, Ik-Hwan; Goo, Sung Young; Park, Soon-Jung; Hwang, Se-Jin; Kim, Yong-Seok; Yang, Michael Sungwoo; Ahn, Myoung-Hee; Ryu, Jae-Sook
- Issue Date
- Sep-2009
- Publisher
- KOREAN SOC PARASITOLOGY, SEOUL NATL UNIV COLL MEDI
- Keywords
- Trichomonas vaginalis; human monocyte-derived macrophage; proinflammatory cytokine; nitric oxide; iNOS; NF-kappa B
- Citation
- KOREAN JOURNAL OF PARASITOLOGY, v.47, no.3, pp.205 - 212
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- KOREAN JOURNAL OF PARASITOLOGY
- Volume
- 47
- Number
- 3
- Start Page
- 205
- End Page
- 212
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176266
- DOI
- 10.3347/kjp.2009.47.3.205
- ISSN
- 0023-4001
- Abstract
- Trichomonas vaginalis commonly causes vaginitis and perhaps cervicitis in women and urethritis in men and women. Macrophages are important immune cells in response to T. vaginalis infection. In this study, we investigated whether human macrophages could be involved in inflammation induced by T. vaginalis. Human monocyte-derived macrophages ( HMDM) were co-cultured with T. vaginalis. Live, opsonized-live trichomonads, and T. vaginalis lysates increased proinflammatory cytokines, such as TNF-alpha, IL-1 beta, and IL-6 by HMDM. The involvement of nuclear factor (NF)-kappa B signaling pathway in cytokine production induced by T. vaginalis was confirmed by phosphorylation and nuclear translocation of p65 NF-kappa B. In addition, stimulation with live T. vaginalis induced marked augmentation of nitric oxide ( NO) production and expression of inducible NO synthase ( iNOS) levels in HMDM. However, trichomonad-induced NF-kappa B activation and TNF-alpha production in macrophages were significantly inhibited by inhibition of iNOS levels with L-NMMA ( NO synthase inhibitor). Moreover, pretreatment with NF-kappa B inhibitors ( PDTC or Bay11-7082) caused human macrophages to produce less TNF-alpha. These results suggest that T. vaginalis stimulates human macrophages to produce proinflammatory cytokines, such as IL-1, IL-6, and TNF-alpha, and NO. In particular, we showed that T. vaginalis induced TNF-alpha production in macrophages through NO-dependent activation of NF-kappa B, which might be closely involved in inflammation caused by T. vaginalis.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 환경의생물학교실 > 1. Journal Articles
- 서울 의과대학 > 서울 생화학·분자생물학교실 > 1. Journal Articles
- 서울 의과대학 > 서울 해부·세포생물학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176266)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.