살모넬라균 검출을 위한 임피던스 바이오센서의 항체 고정화 방법 평가Evaluation of Antibody Immobilization Methods For The Detection of Salmonella Using Impedimetric Biosensor
- Other Titles
- Evaluation of Antibody Immobilization Methods For The Detection of Salmonella Using Impedimetric Biosensor
- Authors
- 김기영; 문지혜; 엄애선; 양길모; 모창연; 강석원; 조한근
- Issue Date
- Aug-2009
- Publisher
- 한국농업기계학회
- Keywords
- Keywords: Impedimetric biosensor; Salmonella; interdigitated microelectrode
- Citation
- Journal of Biosystems Engineering, v.34, no.4, pp.254 - 259
- Indexed
- KCI
- Journal Title
- Journal of Biosystems Engineering
- Volume
- 34
- Number
- 4
- Start Page
- 254
- End Page
- 259
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176334
- DOI
- 10.5307/JBE.2009.34.4.254
- ISSN
- 1738-1266
- Abstract
- Conventional methods for pathogen detection and identification are labor-intensive and take days to complete. Recently developed biosensors have shown potential for the rapid detection of foodborne pathogens. In this study, an impedimetric biosensor was developed for rapid detection of Salmonella typhimurium. To develop the biosensor, an interdigitated microelectrode (IME) was fabricated by using a semiconductor fabrication process. Anti-Salmonella antibodies were immobilized based on either avidin-biotin binding or self assembled monolayer (SAM) on the surface of the IME to form an active sensing layer. To evaluate the effect of antibody immobilization methods on sensitivity of the sensor, detection limit of the biosensor was analyzed with Salmonella samples innoculated in phosphate buffered saline (PBS) or food extract. The impedimetric biosensor based on SAM immobilization method produced better detection limit. The biosensor could detect 107 CFU/mL of Salmonella in pork meat extract. This method may provide a simple, rapid, and sensitive method to detect foodborne pathogens.
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