Effects of intravesical instillation of antimuscarinics on bladder overactivity induced by intravesical instillation of adenosine triphosphate in rats
- Authors
- Lee, Sang Bong; Lee, Jae Won; Kim, Yong Tae
- Issue Date
- Apr-2009
- Publisher
- 대한비뇨의학회
- Keywords
- Adenosine triphosphate; Muscarinic antagonists; Rats
- Citation
- Korean Journal of Urology, v.50, no.4, pp.396 - 400
- Indexed
- SCOPUS
KCI
- Journal Title
- Korean Journal of Urology
- Volume
- 50
- Number
- 4
- Start Page
- 396
- End Page
- 400
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176954
- DOI
- 10.4111/kju.2009.50.4.396
- ISSN
- 2005-6737
- Abstract
- Purpose
Adenosine triphosphate (ATP) from the urothelium acts as a sensory neurotransmitter and is augmented in many diseases, such as overactive bladder. We investigated the effects of intravesical instillation of oxybutynin on ATP-induced bladder overactivity to determine whether this effect is mediated by effects on urothelial muscarinic receptors.
Materials and Methods
Cystometry (at rate of 0.04 ml/min) was performed in female Sprague-Dawley rats (body weight 250 g) under urethane anesthesia (1.2 g/kg). After a 2-hour baseline period, protamine sulfate (10 mg/ml) was instilled for 1 hour, and then ATP (60 mM, pH 6.0) or a mixture of oxybutynin (10-6 M) and ATP (60 mM, pH 6.0) was instilled intravesically. We performed experiments with 4-diphenylacetoxy-N-methylpiperidine methobromide (4-DAMP) and methoctramine by the same methods. Cystometric parameters, such as the intercontraction interval (ICI), pressure threshold (PT), and maximal voiding pressure (MVP), were compared.
Results
With intravesical instillation of ATP after protamine sulfate treatment, the ICI was decreased compared with baseline (ICI: baseline, 487.1±64.8 s; protamine, 450.6±56.1 s; ATP, 229.7±35.3 s; p<0.05). Addition of oxybutynin, 4-DAMP, or methoctramine in the ATP solution did not significantly change the ICI compared with ATP solution alone (ICI: oxybutynin, 189.1±32.3 s; 4-DAMP, 161.1±22.8 s; methoctramine, 341.0±113.3 s; p>0.05). Intravesical instillation of ATP decreased MVP and PT significantly compared with baseline, but MVP and PT were not changed significantly with oxybutynin, 4-DAMP, or methoctramine compared with ATP.
Conclusions
Bladder overactivity induced by intravesical instillation of ATP was not suppressed by intravesical instillation of antimuscarinics. Suppression of ATP-induced bladder overactivity by intravenous oxybutynin is not mediated by urothelial muscarinic receptors.
- Files in This Item
-
Go to Link
- Appears in
Collections - 서울 의과대학 > 서울 비뇨의학교실 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/176954)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.