Delivery of Hypoxia Inducible Heme Oxygenase-1 Gene Using Dexamethasone Conjugated Polyethylenimine for Protection of Cardiomyocytes under Hypoxia
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyunjung | - |
dc.contributor.author | Kim, Hyun Ah | - |
dc.contributor.author | Choi, Joon Sig | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2022-12-20T22:50:44Z | - |
dc.date.available | 2022-12-20T22:50:44Z | - |
dc.date.created | 2022-08-26 | - |
dc.date.issued | 2009-04 | - |
dc.identifier.issn | 0253-2964 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/177036 | - |
dc.description.abstract | Heme oxygenase-1 (HO-1) is an anti-inflammatory and anti-apoptotic protein and has been applied to various gene therapy researches. However, constitutive expression of HO-I may induce deleterious side effects. In this research, hypoxia inducible HO-I expression plasmid, pEpo-SV-HO-1, was constructed with the erythropoietin (epo) enhancer and simian virus 40 (SV40) promoter to avoid these unwanted side effects. Dexamethasone conjugated polyethylenimine (PEI-Dexa) was used as a gene carrier. It was previously reported that dexamethasone protected cardiomyocytes from apoptosis under hypoxia. In this research, PEI-Dexa reduced the caspase-3 level in hypoxic H9C2 cardiomyocytes as a derivative of dexamethasone, suggesting that PEI-Dexa is an anti-apoptotic reagent as well as a gene carrier. pEpo-SV-HO-1 was transfected to H9C2 cardiomyocytes using PEI-Dexa and the cells were incubated under normoxia or hypoxia. HO-1 expression was induced in the pEpo-SV-HO-1 transfected cells under hypoxia. In addition, cell viability under hypoxia was higher in the pEpo-SV-HO-1 transfected cells than the pEpo-SV-Luc transfected cells. Also, caspase-3 level was reduced in the pEpo-SV-HO-1 transfected cells under hypoxia. In addition to the anti-apoptotic effect of PEI-Dexa, hypoxia inducible HO-1 expression by pEpo-SV-HO-1 may be helpful to protect cardiomyocytes under hypoxia. Therefore, pEpo-SV-HO-1/PEI-Dexa complex may be useful for ischemic heart disease gene therapy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Delivery of Hypoxia Inducible Heme Oxygenase-1 Gene Using Dexamethasone Conjugated Polyethylenimine for Protection of Cardiomyocytes under Hypoxia | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunjung | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.5012/bkcs.2009.30.4.897 | - |
dc.identifier.scopusid | 2-s2.0-70349761270 | - |
dc.identifier.wosid | 000266085000029 | - |
dc.identifier.bibliographicCitation | BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.30, no.4, pp.897 - 901 | - |
dc.relation.isPartOf | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.title | BULLETIN OF THE KOREAN CHEMICAL SOCIETY | - |
dc.citation.volume | 30 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 897 | - |
dc.citation.endPage | 901 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001524518 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | Cell death | - |
dc.subject.keywordPlus | Cell membranes | - |
dc.subject.keywordPlus | Gene transfer | - |
dc.subject.keywordPlus | Hemoglobin | - |
dc.subject.keywordPlus | Porphyrins | - |
dc.subject.keywordPlus | Research | - |
dc.subject.keywordPlus | Cardiomyocytes | - |
dc.subject.keywordPlus | Dexamethasone | - |
dc.subject.keywordPlus | Heme oxygenase-1 | - |
dc.subject.keywordPlus | Hypoxia | - |
dc.subject.keywordPlus | Polyethylenimine | - |
dc.subject.keywordAuthor | Dexamethasone | - |
dc.subject.keywordAuthor | Cardiomyocytes | - |
dc.subject.keywordAuthor | Heme oxygenase-1 | - |
dc.subject.keywordAuthor | Hypoxia | - |
dc.subject.keywordAuthor | Polyethylenimine | - |
dc.identifier.url | http://koreascience.or.kr/article/JAKO200902727454503.page | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.